TY - JOUR
KW - Animals
KW - Antigens, Bacterial
KW - Base Sequence
KW - DNA, Bacterial
KW - Gene Amplification
KW - Humans
KW - leprosy
KW - Molecular Sequence Data
KW - Mycobacterium
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Predictive Value of Tests
KW - Species Specificity
AU - Hackel C
AU - Houard S
AU - Portaels F
AU - Elsen A
AU - Herzog A
AU - Bollen A
AB - <p>Oligonucleotide primers have been used to amplify DNA regions of the M. leprae genome by the polymerase chain reaction. A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa antigen of M. leprae. A second pair of primers, targetted to the same gene, leads to the amplification of a 154 bp DNA piece conserved in mycobacteria. Primers PLp1 and PLp2 discriminate the pathogenic species from other mycobacteria, detect down to 40 bacilli, and constitute potentially useful tools for the identification of M. leprae in clinical specimens.</p>
BT - Molecular and cellular probes
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2199822?dopt=Abstract
DA - 1990 Jun
DO - 10.1016/0890-8508(90)90054-4
IS - 3
J2 - Mol. Cell. Probes
LA - eng
N2 - <p>Oligonucleotide primers have been used to amplify DNA regions of the M. leprae genome by the polymerase chain reaction. A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa antigen of M. leprae. A second pair of primers, targetted to the same gene, leads to the amplification of a 154 bp DNA piece conserved in mycobacteria. Primers PLp1 and PLp2 discriminate the pathogenic species from other mycobacteria, detect down to 40 bacilli, and constitute potentially useful tools for the identification of M. leprae in clinical specimens.</p>
PY - 1990
SP - 205
EP - 10
T2 - Molecular and cellular probes
TI - Specific identification of Mycobacterium leprae by the polymerase chain reaction.
VL - 4
SN - 0890-8508
ER -