TY - JOUR
KW - Antibodies, Bacterial
KW - Antibodies, Monoclonal
KW - Antigens, Bacterial
KW - Fluorescent Antibody Technique
KW - Granuloma
KW - Humans
KW - Leprosy, lepromatous
KW - Leprosy, Tuberculoid
KW - Mycobacterium leprae
KW - Peripheral nerves
KW - Skin
AU - Narayanan R B
AU - Girdhar B K
AU - Malaviya G N
AU - Sengupta U
AB - <p>Cryostat sections of skin and nerve lesions of leprosy were stained with monoclonal antibodies recognising Mycobacterium leprae antigens and indirect immunofluorescence. In both the tuberculoid and lepromatous lesions, PGL1, 55-65-kDa, 17-kDa protein antigens and cross-reactive non-protein antigens were present. 65-kDa antigens were seen mainly in the skin lesions of lepromatous leprosy. The infiltrates in both the skin and nerve granulomas of tuberculoid and lepromatous leprosy showed membranous staining with monoclonal antibodies recognising PGL1 and 55-65-kDa antigens. Bacilli in the lesions and the cells in the lymph node granulomas of patients with tuberculosis or the infiltrates in the lesions of tinea corporis or sections of normal skin did not show any staining with these monoclonal antibodies. These results confirm that M. leprae antigens are present and are expressed on the infiltrating cells of leprosy lesions.</p>
BT - Immunology letters
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2200752?dopt=Abstract
DA - 1990 Jun
DO - 10.1016/0165-2478(90)90045-r
IS - 3
J2 - Immunol. Lett.
LA - eng
N2 - <p>Cryostat sections of skin and nerve lesions of leprosy were stained with monoclonal antibodies recognising Mycobacterium leprae antigens and indirect immunofluorescence. In both the tuberculoid and lepromatous lesions, PGL1, 55-65-kDa, 17-kDa protein antigens and cross-reactive non-protein antigens were present. 65-kDa antigens were seen mainly in the skin lesions of lepromatous leprosy. The infiltrates in both the skin and nerve granulomas of tuberculoid and lepromatous leprosy showed membranous staining with monoclonal antibodies recognising PGL1 and 55-65-kDa antigens. Bacilli in the lesions and the cells in the lymph node granulomas of patients with tuberculosis or the infiltrates in the lesions of tinea corporis or sections of normal skin did not show any staining with these monoclonal antibodies. These results confirm that M. leprae antigens are present and are expressed on the infiltrating cells of leprosy lesions.</p>
PY - 1990
SP - 179
EP - 83
T2 - Immunology letters
TI - In situ demonstration of Mycobacterium leprae antigens in leprosy lesions using monoclonal antibodies.
VL - 24
SN - 0165-2478
ER -