TY - JOUR
KW - DNA, Bacterial
KW - Diagnostic Errors
KW - Evaluation Studies as Topic
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Skin
AU - Wit M Y
AU - Faber W R
AU - Krieg S R
AU - Douglas J T
AU - Lucas S B
AU - Montreewasuwat N
AU - Pattyn S R
AU - Hussain R
AU - Ponnighaus J M
AU - Hartskeerl R A
AB - <p>The polymerase chain reaction (PCR) based on the selective amplification of a 530-bp fragment of the gene encoding the proline-rich antigen of Mycobacterium leprae was applied on sections of fixed or frozen biopsy samples from leprosy patients. A simple procedure for the extraction of DNA from M. leprae in clinical specimens that provided suitable template DNA for amplification was developed. When PCR was applied on frozen sections, positive amplification in samples from all untreated acid-fast bacillus (AFB)-positive patients and in samples from 56% of the untreated AFB-negative patients could be detected, while biopsy samples from patients with skin diseases other than leprosy were all PCR negative. With neutral Formalin-fixed biopsy samples, positive amplification in 92% of the samples from untreated AFB-positive patients and in 61% of the samples from untreated AFB-negative patients could be detected by PCR. Biopsy samples exposed to mercuric chloride or nonbuffered formaldehyde containing fixatives were not suitable for application of PCR. This PCR holds promise as a tool for studies on M. leprae infection.</p>
BT - Journal of clinical microbiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2056057?dopt=Abstract
DA - 1991 May
DO - 10.1136/bmj.c3651
IS - 5
J2 - J. Clin. Microbiol.
LA - eng
N2 - <p>The polymerase chain reaction (PCR) based on the selective amplification of a 530-bp fragment of the gene encoding the proline-rich antigen of Mycobacterium leprae was applied on sections of fixed or frozen biopsy samples from leprosy patients. A simple procedure for the extraction of DNA from M. leprae in clinical specimens that provided suitable template DNA for amplification was developed. When PCR was applied on frozen sections, positive amplification in samples from all untreated acid-fast bacillus (AFB)-positive patients and in samples from 56% of the untreated AFB-negative patients could be detected, while biopsy samples from patients with skin diseases other than leprosy were all PCR negative. With neutral Formalin-fixed biopsy samples, positive amplification in 92% of the samples from untreated AFB-positive patients and in 61% of the samples from untreated AFB-negative patients could be detected by PCR. Biopsy samples exposed to mercuric chloride or nonbuffered formaldehyde containing fixatives were not suitable for application of PCR. This PCR holds promise as a tool for studies on M. leprae infection.</p>
PY - 1991
SP - 906
EP - 10
T2 - Journal of clinical microbiology
TI - Application of a polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues.
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC269906/pdf/jcm00041-0085.pdf
VL - 29
SN - 0095-1137
ER -