TY - JOUR
KW - Antigens, CD
KW - Antigens, Differentiation, Myelomonocytic
KW - Apoptosis
KW - Biopsy
KW - Caspase 3
KW - Humans
KW - Immunohistochemistry
KW - leprosy
KW - Macrophages
KW - Microscopy
KW - Muramidase
KW - Nitric Oxide Synthase Type II
KW - Skin
AU - Quaresma JAS
AU - Lima LWO
AU - Fuzii HT
AU - Libonati RMF
AU - Pagliari C
AU - Duarte MIS
AB - <p>The objective of the present study was to investigate the correlation between macrophage activity and apoptosis in the polar forms of leprosy because the immunopathological phenomena involved in these forms are still poorly understood. For this purpose, 29 skin biopsy samples obtained from patients with the polar forms of leprosy were analyzed. Macrophage activity and apoptosis were evaluated by immunohistochemistry using lysozyme, CD68, iNOS and caspase 3 as markers. The nonparametric Mann-Whitney test and Spearman's linear correlation test were used for statistical analysis. The results suggest that the apoptosis rate is under the direct influence of macrophage activity in lesions of patients with the tuberculoid form. In contrast, in lepromatous lesions other factors seem to induce programmed cell death, possibly TGF-beta. Further studies are necessary to identify additional factors involved in the immunopathogenesis of leprosy.</p>
BT - Microbial pathogenesis
C1 - http://www.ncbi.nlm.nih.gov/pubmed/20510345?dopt=Abstract
DA - 2010 Oct
DO - 10.1016/j.micpath.2010.05.003
IS - 4
J2 - Microb. Pathog.
LA - eng
N2 - <p>The objective of the present study was to investigate the correlation between macrophage activity and apoptosis in the polar forms of leprosy because the immunopathological phenomena involved in these forms are still poorly understood. For this purpose, 29 skin biopsy samples obtained from patients with the polar forms of leprosy were analyzed. Macrophage activity and apoptosis were evaluated by immunohistochemistry using lysozyme, CD68, iNOS and caspase 3 as markers. The nonparametric Mann-Whitney test and Spearman's linear correlation test were used for statistical analysis. The results suggest that the apoptosis rate is under the direct influence of macrophage activity in lesions of patients with the tuberculoid form. In contrast, in lepromatous lesions other factors seem to induce programmed cell death, possibly TGF-beta. Further studies are necessary to identify additional factors involved in the immunopathogenesis of leprosy.</p>
PY - 2010
SP - 135
EP - 40
T2 - Microbial pathogenesis
TI - Immunohistochemical evaluation of macrophage activity and its relationship with apoptotic cell death in the polar forms of leprosy.
VL - 49
SN - 1096-1208
ER -