TY - JOUR
KW - Antigens, Bacterial
KW - Enzyme-Linked Immunosorbent Assay
KW - Glycolipids
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - Sensitivity and Specificity
AU - Mahon A C
AU - Nurlign A
AU - Kebede B
AU - Becx-Bleumink M
AU - Lefford M J
AB - <p>A simplified assay to measure the phenolic glycolipid 1 (PGL-1) of Mycobacterium leprae in the urine was applied to the diagnosis of leprosy and the monitoring of antileprosy chemotherapy. One hundred seventy-nine previously untreated patients and 25 normal controls were tested. The specificity of the assay was 100%. There were no false-positive results. The sensitivity of the assay varied with the type of leprosy from 92% for lepromatous leprosy to 56% for borderline lepromatous and 18% for borderline tuberculoid patients. After the onset of chemotherapy in lepromatous leprosy patients, there was often a transient increase of urinary PGL-1, followed by a steady decline. Within 3 months of multiple drug therapy, urinary PGL-1 levels were reduced by 90%-99% and were often undetectable. This assay appears to have considerable potential for monitoring chemotherapy and detecting treatment failure and relapse in patients with Hansen's disease.</p>
BT - The Journal of infectious diseases
C1 - http://www.ncbi.nlm.nih.gov/pubmed/1995739?dopt=Abstract
DA - 1991 Mar
DO - 10.1093/infdis/163.3.653
IS - 3
J2 - J. Infect. Dis.
LA - eng
N2 - <p>A simplified assay to measure the phenolic glycolipid 1 (PGL-1) of Mycobacterium leprae in the urine was applied to the diagnosis of leprosy and the monitoring of antileprosy chemotherapy. One hundred seventy-nine previously untreated patients and 25 normal controls were tested. The specificity of the assay was 100%. There were no false-positive results. The sensitivity of the assay varied with the type of leprosy from 92% for lepromatous leprosy to 56% for borderline lepromatous and 18% for borderline tuberculoid patients. After the onset of chemotherapy in lepromatous leprosy patients, there was often a transient increase of urinary PGL-1, followed by a steady decline. Within 3 months of multiple drug therapy, urinary PGL-1 levels were reduced by 90%-99% and were often undetectable. This assay appears to have considerable potential for monitoring chemotherapy and detecting treatment failure and relapse in patients with Hansen's disease.</p>
PY - 1991
SP - 653
EP - 6
T2 - The Journal of infectious diseases
TI - Urinary phenolic glycolipid 1 in the diagnosis and management of leprosy.
VL - 163
SN - 0022-1899
ER -