TY - JOUR
KW - CD4-Positive T-Lymphocytes
KW - Dendritic Cells
KW - Dinitrochlorobenzene
KW - Foam Cells
KW - Granuloma
KW - HLA-DR Antigens
KW - Humans
KW - Hypersensitivity, Delayed
KW - Immunity, Cellular
KW - Immunohistochemistry
KW - Interferon-gamma
KW - Keratinocytes
KW - Leprosy, lepromatous
KW - Leprosy, Tuberculoid
KW - Macrophages
KW - Receptors, Interleukin-2
KW - T-Lymphocytes, Helper-Inducer
KW - T-Lymphocytes, Regulatory
AU - Volc-Platzer B
AU - Kremsner P
AU - Stemberger H
AU - Wiedermann G
AB - <p>Immunohistological studies of tuberculoid leprosy lesions (TT-lesions) showed a dense, well organized granuloma consisting of a central area with epitheloid and giant cells containing interferon-gamma (IFN-Gamma) and CD3+, CD4+ T helper/inducer (Th/i) cells, a considerable proportion of which expressed the interleukin-2-receptor (IL-2 R). This central area was surrounded by round cells which consisted mainly of CD3+/CD8+ T cytotoxic/suppressor (Tc/s) lymphocytes. The overlying keratinocytes (KC) were strongly positive for HLA-DR antigens on the surface, indicating high intralesional IFN-Gamma activity. In contrast, lepromatous leprosy lesions (LL-lesions) showed a disorganized infiltrate composed by foamy cells and round cells, the latter mainly expressing the CD3+/CD8+ phenotype. IFN-Gamma activity could not be detected within the lesions. The KC overlying the infiltrate were consistently negative for HLA/DR reactivity pointing to a defective intralesional IFN-Gamma production in LL patients. Two out of four patients with LL leprosy could be sensitized with dinitrochlorobenzene (DNCB). The eliciting of DNCB skin reactions within the LL-lesion led to the recruitment of new infiltrating cells; the resulting infiltrate resembled a local reversal towards the tuberculoid pole of leprosy.</p>
BT - Zentralblatt fur Bakteriologie : international journal of medical microbiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/1972881?dopt=Abstract
DA - 1990 Apr
DO - 10.1016/s0934-8840(11)80047-2
IS - 4
J2 - Zentralbl. Bakteriol.
LA - eng
N2 - <p>Immunohistological studies of tuberculoid leprosy lesions (TT-lesions) showed a dense, well organized granuloma consisting of a central area with epitheloid and giant cells containing interferon-gamma (IFN-Gamma) and CD3+, CD4+ T helper/inducer (Th/i) cells, a considerable proportion of which expressed the interleukin-2-receptor (IL-2 R). This central area was surrounded by round cells which consisted mainly of CD3+/CD8+ T cytotoxic/suppressor (Tc/s) lymphocytes. The overlying keratinocytes (KC) were strongly positive for HLA-DR antigens on the surface, indicating high intralesional IFN-Gamma activity. In contrast, lepromatous leprosy lesions (LL-lesions) showed a disorganized infiltrate composed by foamy cells and round cells, the latter mainly expressing the CD3+/CD8+ phenotype. IFN-Gamma activity could not be detected within the lesions. The KC overlying the infiltrate were consistently negative for HLA/DR reactivity pointing to a defective intralesional IFN-Gamma production in LL patients. Two out of four patients with LL leprosy could be sensitized with dinitrochlorobenzene (DNCB). The eliciting of DNCB skin reactions within the LL-lesion led to the recruitment of new infiltrating cells; the resulting infiltrate resembled a local reversal towards the tuberculoid pole of leprosy.</p>
PY - 1990
SP - 458
EP - 66
T2 - Zentralblatt fur Bakteriologie : international journal of medical microbiology
TI - Restoration of defective cytokine activity within lepromatous leprosy lesions.
VL - 272
SN - 0934-8840
ER -