TY - JOUR
KW - CD4-Positive T-Lymphocytes
KW - Cells, Cultured
KW - Child, Preschool
KW - Granulocyte-Macrophage Colony-Stimulating Factor
KW - Humans
KW - Infant
KW - Infant, Newborn
KW - Interleukin-10
KW - Lymphocyte Activation
KW - Macrophages
KW - Membrane Proteins
KW - Mycobacterium bovis
AU - Makino M
AU - Maeda Y
AU - Kai M
AU - Tamura T
AU - Mukai T
AB - <p>The potential of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) needs to be augmented to efficiently activate CD4(+) T cells through macrophages. Mycobacterium leprae-derived recombinant major membrane protein (MMP)-II induced GM-CSF production from macrophages. A recombinant BCG-SM that secretes MMP-II more efficiently produced GM-CSF and activated interferon (IFN)-gamma-producing CD4(+) T cells than did vector control BCG when infected with macrophages. The T-cell activation by BCG-SM was dependent on the GM-CSF production by macrophages. Interleukin (IL)-10 production by macrophages stimulated with M. leprae was inhibited in a GM-CSF-dependent manner when the precursor monocytes were infected with BCG-SM. BCG inducing GM-CSF production was effective in macrophage-mediated T-cell activation partially through IL-10 inhibition.</p>
BT - FEMS immunology and medical microbiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/19076223?dopt=Abstract
DA - 2009 Jan
DO - 10.1111/j.1574-695X.2008.00495.x
IS - 1
J2 - FEMS Immunol. Med. Microbiol.
LA - eng
N2 - <p>The potential of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) needs to be augmented to efficiently activate CD4(+) T cells through macrophages. Mycobacterium leprae-derived recombinant major membrane protein (MMP)-II induced GM-CSF production from macrophages. A recombinant BCG-SM that secretes MMP-II more efficiently produced GM-CSF and activated interferon (IFN)-gamma-producing CD4(+) T cells than did vector control BCG when infected with macrophages. The T-cell activation by BCG-SM was dependent on the GM-CSF production by macrophages. Interleukin (IL)-10 production by macrophages stimulated with M. leprae was inhibited in a GM-CSF-dependent manner when the precursor monocytes were infected with BCG-SM. BCG inducing GM-CSF production was effective in macrophage-mediated T-cell activation partially through IL-10 inhibition.</p>
PY - 2009
SP - 39
EP - 46
T2 - FEMS immunology and medical microbiology
TI - GM-CSF-mediated T-cell activation by macrophages infected with recombinant BCG that secretes major membrane protein-II of Mycobacterium leprae.
VL - 55
SN - 1574-695X
ER -