TY - JOUR
KW - Animals
KW - Complement Activation
KW - Erythema Nodosum
KW - Humans
KW - Leprosy, lepromatous
KW - Mice
KW - Mice, Nude
KW - Mycobacterium leprae
KW - Sonication
AU - Lahiri R
AU - Sandoval FG
AU - Krahenbuhl JL
AU - Shannon E
AB - <p><b>OBJECTIVE: </b>The immune-mediated events that precipitate erythema nodosum leprosum (ENL) are not well understood. One component may be the complexing of antibody with antigens released from infected macrophages, the activation of complement and the subsequent local inflammation. We assess here the ability of highly-purified, disrupted M. leprae, to activate complement.</p><p><b>RESULTS: </b>Intact and sonically-disrupted crude and alkali-purified nu/nu mouse-derived M. leprae suspensions were incubated with normal serum and a hemolytic titer (CH50) was determined as a measure of complement fixation. Crude M. leprae consumed complement, and disrupted preparations more than the intact. Purified M. leprae preparations did not consume complement unless disrupted.</p><p><b>CONCLUSION: </b>M. leprae, if disrupted, can activate complement. This supports a hypothesis that links released antigens with ENL, and may explain the increased probability of an occurrence of ENL following chemotherapy.</p>
BT - Leprosy review
C1 - http://www.ncbi.nlm.nih.gov/pubmed/19009980?dopt=Abstract
CN - Infolep Library - available
DA - 2008 Sep
IS - 3
J2 - Lepr Rev
LA - eng
N2 - <p><b>OBJECTIVE: </b>The immune-mediated events that precipitate erythema nodosum leprosum (ENL) are not well understood. One component may be the complexing of antibody with antigens released from infected macrophages, the activation of complement and the subsequent local inflammation. We assess here the ability of highly-purified, disrupted M. leprae, to activate complement.</p><p><b>RESULTS: </b>Intact and sonically-disrupted crude and alkali-purified nu/nu mouse-derived M. leprae suspensions were incubated with normal serum and a hemolytic titer (CH50) was determined as a measure of complement fixation. Crude M. leprae consumed complement, and disrupted preparations more than the intact. Purified M. leprae preparations did not consume complement unless disrupted.</p><p><b>CONCLUSION: </b>M. leprae, if disrupted, can activate complement. This supports a hypothesis that links released antigens with ENL, and may explain the increased probability of an occurrence of ENL following chemotherapy.</p>
PY - 2008
SP - 311
EP - 4
T2 - Leprosy review
TI - Activation of complement by Mycobacterium leprae requires disruption of the bacilli.
UR - https://leprosyreview.org/article/79/3/31-1314
VL - 79
SN - 0305-7518
ER -