The sugar content of the trisaccharide-BSA conjugate of the phenolic glycolip I of Mycobacterium leprae (NT-P-BSA) increased with the increase of the molar ratio of the trisaccharide to BSA used in the coupling reaction. The difference of the sugar content in NT-P-BSA did not give the influence on the seroreactivity and specificity in ELISA for both IgM and IgG class antibodies. During the course of the coupling reaction, about half amount of BSA was converted to dimeric form. However, there were no differences of the activity and specificity between monomeric form and dimeric form of NT-P-BSA. Based on these results, it was concluded that any lot of NT-P-BSA with variety of sugar content can be used in ELISA without any difference of the seroreactivity.
BT - Nihon Rai Gakkai zasshi C1 - http://www.ncbi.nlm.nih.gov/pubmed/1843226?dopt=Abstract DA - 1991 Jul-Dec DO - 10.5025/hansen1977.60.132 IS - 3-4 J2 - Nihon Rai Gakkai Zasshi LA - eng N2 -The sugar content of the trisaccharide-BSA conjugate of the phenolic glycolip I of Mycobacterium leprae (NT-P-BSA) increased with the increase of the molar ratio of the trisaccharide to BSA used in the coupling reaction. The difference of the sugar content in NT-P-BSA did not give the influence on the seroreactivity and specificity in ELISA for both IgM and IgG class antibodies. During the course of the coupling reaction, about half amount of BSA was converted to dimeric form. However, there were no differences of the activity and specificity between monomeric form and dimeric form of NT-P-BSA. Based on these results, it was concluded that any lot of NT-P-BSA with variety of sugar content can be used in ELISA without any difference of the seroreactivity.
PY - 1991 SP - 132 EP - 8 T2 - Nihon Rai Gakkai zasshi TI - Does the difference of the properties of trisaccharide-BSA conjugate (NT-P-BSA) of Mycobacterium leprae phenolic glycolipid influence on its seroreactivity? VL - 60 SN - 0386-3980 ER -