TY - JOUR KW - Antigens, Bacterial KW - Contact Tracing KW - Enzyme-Linked Immunosorbent Assay KW - Glycolipids KW - Humans KW - Immunoblotting KW - Immunoglobulin M KW - leprosy KW - Predictive Value of Tests KW - Skin AU - Saad M H AU - Medeiros M A AU - Gallo M E AU - Gontijo-Filho P P AU - Fonseca L S AB -

1. Since dot-ELISA has recently been reported to be a sensitive, simple and fast method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. 2. The sensitivity of the two techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93.4%). 3. Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. 4. These data indicate that dot-ELISA has a high specificity and can be a useful tool in field evaluation.

BT - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas C1 - http://www.ncbi.nlm.nih.gov/pubmed/1823258?dopt=Abstract DA - 1991 IS - 5 J2 - Braz. J. Med. Biol. Res. LA - eng N2 -

1. Since dot-ELISA has recently been reported to be a sensitive, simple and fast method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. 2. The sensitivity of the two techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93.4%). 3. Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. 4. These data indicate that dot-ELISA has a high specificity and can be a useful tool in field evaluation.

PY - 1991 SP - 441 EP - 8 T2 - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas TI - The dot-ELISA test for the detection of anti-PGL-1 IgM in leprosy patients and their contacts. VL - 24 SN - 0100-879X ER -