TY - JOUR
KW - Alleles
KW - Argentina
KW - Female
KW - HLA-DR Antigens
KW - HLA-DRB1 Chains
KW - Humans
KW - leprosy
KW - Male
KW - Middle Aged
AU - Borrás SG
AU - Cotorruelo C
AU - Racca L
AU - Recarte M
AU - Garcías C
AU - Biondi C
AU - Bottasso O
AU - Racca A
AB - <p><b>BACKGROUND: </b>Previous studies have suggested an influence of HLA molecules on the regulation of the anti Mycobacterium leprae immune response.</p><p><b>METHODS: </b>DNA typing of HLA-DRB1 alleles in 71 leprosy patients and 81 healthy controls was performed. Genomic DNA was extracted from peripheral blood and used as a template to amplify the polymorphic second exon of the HLA-DRB1 by the polymerase chain reaction (PCR). PCR products were hybridized separately with sequence-specific oligonucleotides.</p><p><b>RESULTS: </b>DRB1*1401 and DRB1*1406 alleles were significantly more prevalent in leprosy patients, whereas a decreased frequency of DRB1*0808 and DRB1*1103 alleles was found, by comparison with the group control.</p><p><b>CONCLUSIONS: </b>The HLA-DRB1 alleles could act alone or in combination with other genes to confer differential susceptibility and also protection to leprosy disease in endemic areas of the American continent.</p>
BT - Annals of clinical biochemistry
C1 - http://www.ncbi.nlm.nih.gov/pubmed/18275683?dopt=Abstract
CN - BORRAS2008
DA - 2008 Jan
DO - 10.1258/acb.2007.007156
IS - Pt 1
J2 - Ann. Clin. Biochem.
LA - eng
N2 - <p><b>BACKGROUND: </b>Previous studies have suggested an influence of HLA molecules on the regulation of the anti Mycobacterium leprae immune response.</p><p><b>METHODS: </b>DNA typing of HLA-DRB1 alleles in 71 leprosy patients and 81 healthy controls was performed. Genomic DNA was extracted from peripheral blood and used as a template to amplify the polymorphic second exon of the HLA-DRB1 by the polymerase chain reaction (PCR). PCR products were hybridized separately with sequence-specific oligonucleotides.</p><p><b>RESULTS: </b>DRB1*1401 and DRB1*1406 alleles were significantly more prevalent in leprosy patients, whereas a decreased frequency of DRB1*0808 and DRB1*1103 alleles was found, by comparison with the group control.</p><p><b>CONCLUSIONS: </b>The HLA-DRB1 alleles could act alone or in combination with other genes to confer differential susceptibility and also protection to leprosy disease in endemic areas of the American continent.</p>
PY - 2008
SP - 96
EP - 8
T2 - Annals of clinical biochemistry
TI - Association of leprosy with HLA-DRB1 in an Argentinean population.
UR - http://acb.sagepub.com/content/45/1/96.full.pdf+html
VL - 45
SN - 0004-5632
ER -