TY - JOUR KW - Antibodies, Bacterial KW - Cells, Cultured KW - Cytokines KW - Dose-Response Relationship, Immunologic KW - Enzyme-Linked Immunosorbent Assay KW - Granulocyte-Macrophage Colony-Stimulating Factor KW - Humans KW - Immune Sera KW - Immunity, Cellular KW - Interferon-gamma KW - leprosy KW - Leprosy, lepromatous KW - Leprosy, Tuberculoid KW - Monocytes KW - Mycobacterium bovis KW - Mycobacterium leprae KW - Phagocytosis KW - Recombinant Proteins AU - Launois P AU - Blum L AU - Dieye A AU - Millan J AU - Sarthou J L AU - Michel J C AU - Bach M A AB -

Mycobacterium leprae, in contrast to BCG, failed to trigger any chemiluminescence (CL) response in mononuclear cells from either leprosy patients or healthy subjects, a deficit not reversed by either interferon-gamma or GM-CSF. Chemiluminescence responses induced without mycobacteria or with BCG were found to be lower in leprosy patients than in controls. M. leprae were also less well phagocytosed than BCG. However, there was a significant difference in phagocytosis between healthy and tuberculoid leprosy subjects. Phagocytosis was not altered by the addition of either lymphokine, and no major differences between healthy subjects and patients were observed. Preincubating mononuclear cells with anti-mycobacteria antibodies (lepromatous patients' sera) did not increase the CL response nor the phagocytosis of M. leprae or BCG.

BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -

http://www.ncbi.nlm.nih.gov/pubmed/1802941?dopt=Abstract

DA - 1991 Dec IS - 4 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -

Mycobacterium leprae, in contrast to BCG, failed to trigger any chemiluminescence (CL) response in mononuclear cells from either leprosy patients or healthy subjects, a deficit not reversed by either interferon-gamma or GM-CSF. Chemiluminescence responses induced without mycobacteria or with BCG were found to be lower in leprosy patients than in controls. M. leprae were also less well phagocytosed than BCG. However, there was a significant difference in phagocytosis between healthy and tuberculoid leprosy subjects. Phagocytosis was not altered by the addition of either lymphokine, and no major differences between healthy subjects and patients were observed. Preincubating mononuclear cells with anti-mycobacteria antibodies (lepromatous patients' sera) did not increase the CL response nor the phagocytosis of M. leprae or BCG.

PY - 1991 SP - 582 EP - 9 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - M. leprae- and BCG-induced chemiluminescence response of monocytes from leprosy patients and healthy subjects: effects of gamma-interferon and GM-CSF. UR - http://ila.ilsl.br/pdfs/v59n4a06.pdf VL - 59 SN - 0148-916X ER -