TY - JOUR
KW - Adolescent
KW - Child
KW - Female
KW - Humans
KW - In Situ Hybridization
KW - leprosy
KW - Male
KW - polymerase chain reaction
KW - Skin
AU - Dayal R
AU - Agarwal M
AU - Natrajan M
AU - Katoch V M
AU - Katoch K
AU - Singh K
AU - Chauhan D S
AB - <p><b>OBJECTIVE: </b>To assess the diagnostic value of Polymerase Chain Reaction (PCR) and in situ hybridization.</p><p><b>METHODS: </b>This prospective study was carried out in 22 patients</p><p><b>RESULTS: </b>The histopathological examination confirmed the clinical diagnosis in 27.2% cases only. In situ hybridization showed a positivity of 42.8% in early (I/BT) and 46.7% in BB/BL group. In situ hybridization thus enhanced the diagnosis by 18.1%. PCR targeting 36 kDa gene of M. leprae was performed on 15 cases. In these 15 cases, histopathology confirmed the diagnosis in 4 cases (26.6%) and PCR confirmed the diagnosis in 10 cases (66.6%), thus enhancing the diagnosis by 40%.</p><p><b>CONCLUSION: </b>36 kDa PCR and in situ hybridization enhance the diagnosis of leprosy when compared to routine histopathology. They are important diagnostic tools for definitive diagnosis in early and doubtful cases of leprosy.</p>
BT - Indian journal of pediatrics
C1 - http://www.ncbi.nlm.nih.gov/pubmed/17699973?dopt=Abstract
DA - 2007 Jul
DO - 10.1007/s12098-007-0115-x
IS - 7
J2 - Indian J Pediatr
LA - eng
N2 - <p><b>OBJECTIVE: </b>To assess the diagnostic value of Polymerase Chain Reaction (PCR) and in situ hybridization.</p><p><b>METHODS: </b>This prospective study was carried out in 22 patients</p><p><b>RESULTS: </b>The histopathological examination confirmed the clinical diagnosis in 27.2% cases only. In situ hybridization showed a positivity of 42.8% in early (I/BT) and 46.7% in BB/BL group. In situ hybridization thus enhanced the diagnosis by 18.1%. PCR targeting 36 kDa gene of M. leprae was performed on 15 cases. In these 15 cases, histopathology confirmed the diagnosis in 4 cases (26.6%) and PCR confirmed the diagnosis in 10 cases (66.6%), thus enhancing the diagnosis by 40%.</p><p><b>CONCLUSION: </b>36 kDa PCR and in situ hybridization enhance the diagnosis of leprosy when compared to routine histopathology. They are important diagnostic tools for definitive diagnosis in early and doubtful cases of leprosy.</p>
PY - 2007
SP - 645
EP - 8
T2 - Indian journal of pediatrics
TI - PCR and in-situ hybridization for diagnosis of leprosy.
VL - 74
SN - 0973-7693
ER -