As serodiagnosis is the easiest way of diagnosing a disease, the utility of Mycobacterium leprae-derived major membrane protein-II (MMP-II), one of the immuno-dominant antigens, in the serodiagnosis of leprosy was examined. The percent positivity by an enzyme-linked immunosorbent assay for anti-MMP-II antibody was 82.4% for multi-bacillary leprosy, and the specificity of the test was 90.1%. For pauci-bacillary leprosy where cell-mediated immunity predominates, 39.0% showed positive results. These percentage values were significantly higher than these values obtained for existing phenolic glycolipid-I based methods, suggesting that MMP-II antibody detection would facilitate the diagnosis of leprosy.
BT - FEMS microbiology letters C1 - http://www.ncbi.nlm.nih.gov/pubmed/17521364?dopt=Abstract CN - MAEDA 2007 DA - 2007 Jul DO - 10.1111/j.1574-6968.2007.00754.x IS - 2 J2 - FEMS Microbiol. Lett. LA - eng N2 -As serodiagnosis is the easiest way of diagnosing a disease, the utility of Mycobacterium leprae-derived major membrane protein-II (MMP-II), one of the immuno-dominant antigens, in the serodiagnosis of leprosy was examined. The percent positivity by an enzyme-linked immunosorbent assay for anti-MMP-II antibody was 82.4% for multi-bacillary leprosy, and the specificity of the test was 90.1%. For pauci-bacillary leprosy where cell-mediated immunity predominates, 39.0% showed positive results. These percentage values were significantly higher than these values obtained for existing phenolic glycolipid-I based methods, suggesting that MMP-II antibody detection would facilitate the diagnosis of leprosy.
PY - 2007 SP - 202 EP - 5 T2 - FEMS microbiology letters TI - Evaluation of major membrane protein-II as a tool for serodiagnosis of leprosy. UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2007.00754.x/pdf VL - 272 SN - 0378-1097 ER -