TY - JOUR
KW - Animals
KW - Dapsone
KW - Drug Resistance, Microbial
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Mice
KW - Microbial Sensitivity Tests
KW - Mutation
KW - Mycobacterium leprae
KW - Ofloxacin
KW - Oligonucleotide Array Sequence Analysis
KW - Rifampin
AU - Suzuki Y
AU - Matsuoka M
AB - <p>Antibiotic susceptibility test of Mycobacterium leprae still relies on the time consuming methods based on the growth of M. leprae in the mouse footpad. Thus, the establishment of a rapid, simple and reliable method for the detection of drug-resistant M. leprae is one of the most urgent subjects in the treatment of leprosy patients. Recently, many data on the mutation of specific genes correlating with drug resistance have been accumulated. Application of these data permit the establishment of new gene diagnostic methods for drug susceptibility test of leprosy. In this paper, the method using the low density oligonucleotide array that enables the detection of base substitutions involved in resistance against anti-leprosy drugs on a single platform was discussed. The low density oligonucleotide array described in this paper will open the new perspectives in terms of patient management for leprosy with low cost requirement.</p>
BT - Nihon Hansenbyo Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association
C1 - http://www.ncbi.nlm.nih.gov/pubmed/17037383?dopt=Abstract
DA - 2006 Sep
IS - 3
J2 - Nihon Hansenbyo Gakkai Zasshi
LA - jpn
N2 - <p>Antibiotic susceptibility test of Mycobacterium leprae still relies on the time consuming methods based on the growth of M. leprae in the mouse footpad. Thus, the establishment of a rapid, simple and reliable method for the detection of drug-resistant M. leprae is one of the most urgent subjects in the treatment of leprosy patients. Recently, many data on the mutation of specific genes correlating with drug resistance have been accumulated. Application of these data permit the establishment of new gene diagnostic methods for drug susceptibility test of leprosy. In this paper, the method using the low density oligonucleotide array that enables the detection of base substitutions involved in resistance against anti-leprosy drugs on a single platform was discussed. The low density oligonucleotide array described in this paper will open the new perspectives in terms of patient management for leprosy with low cost requirement.</p>
PY - 2006
SP - 271
EP - 7
T2 - Nihon Hansenbyo Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association
TI - [DNA microarray based rapid drug susceptibility test for Mycobacterium leprae].
VL - 75
SN - 1342-3681
ER -