TY - JOUR KW - Antigens, Bacterial KW - Base Sequence KW - CD4-Positive T-Lymphocytes KW - Cells, Cultured KW - Clone Cells KW - Complementarity Determining Regions KW - HLA-DR Antigens KW - Histocompatibility Testing KW - Humans KW - Interferon-gamma KW - leprosy KW - Male KW - Molecular Sequence Data KW - Mycobacterium leprae KW - Receptors, Antigen, T-Cell, alpha-beta KW - Reverse Transcriptase Polymerase Chain Reaction AU - Sabet S AU - Ochoa M AU - Sieling PA AU - Rea T AU - Modlin RL AB -

Human infection with Mycobacterium leprae, an intracellular bacterium, presents as a clinical and immunological spectrum; thus leprosy provides an opportunity to investigate mechanisms of T-cell responsiveness to a microbial pathogen. Analysis of the T-cell receptor (TCR) repertoire in leprosy lesions revealed that TCR BV6(+) T cells containing a conserved CDR3 motif are over-represented in lesions from patients with the localized form of the disease. Here, we derived a T-cell clone from a leprosy lesion that expressed TCR BV6 and the conserved CDR3 sequence L-S-G. This T-cell clone produced a T helper type 1 cytokine pattern, directly lysed M. leprae-pulsed antigen-presenting cells by the granule exocytosis pathway, and expressed the antimicrobial protein granulysin. BV6(+) T cells may therefore functionally contribute to the cell-mediated immune response against M. leprae.

BT - Immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/17140401?dopt=Abstract DA - 2007 Mar DO - 10.1111/j.1365-2567.2006.02510.x IS - 3 J2 - Immunology LA - eng N2 -

Human infection with Mycobacterium leprae, an intracellular bacterium, presents as a clinical and immunological spectrum; thus leprosy provides an opportunity to investigate mechanisms of T-cell responsiveness to a microbial pathogen. Analysis of the T-cell receptor (TCR) repertoire in leprosy lesions revealed that TCR BV6(+) T cells containing a conserved CDR3 motif are over-represented in lesions from patients with the localized form of the disease. Here, we derived a T-cell clone from a leprosy lesion that expressed TCR BV6 and the conserved CDR3 sequence L-S-G. This T-cell clone produced a T helper type 1 cytokine pattern, directly lysed M. leprae-pulsed antigen-presenting cells by the granule exocytosis pathway, and expressed the antimicrobial protein granulysin. BV6(+) T cells may therefore functionally contribute to the cell-mediated immune response against M. leprae.

PY - 2007 SP - 354 EP - 61 T2 - Immunology TI - Functional characterization of a T-cell receptor BV6+ T-cell clone derived from a leprosy lesion. UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265884/pdf/imm0120-0354.pdf VL - 120 SN - 0019-2805 ER -