TY - JOUR
KW - Biological Assay
KW - Biopsy
KW - Drug Resistance, Bacterial
KW - Genes, Bacterial
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Rifampin
AU - Sapkota BR
AU - Ranjit C
AU - Macdonald M
AB - <p>Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.</p>
BT - Nepal Medical College journal : NMCJ
C1 - http://www.ncbi.nlm.nih.gov/pubmed/17017404?dopt=Abstract
DA - 2006 Jun
IS - 2
J2 - Nepal Med Coll J
LA - eng
N2 - <p>Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.</p>
PY - 2006
SP - 122
EP - 7
T2 - Nepal Medical College journal : NMCJ
TI - Reverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.
VL - 8
ER -