TY - JOUR
KW - Amino Acid Sequence
KW - Animals
KW - Antibodies, Bacterial
KW - Antigens, Bacterial
KW - Cells, Cultured
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Immunologic Tests
KW - Interferon-gamma
KW - leprosy
KW - Leukocytes, Mononuclear
KW - Mice
KW - Mice, Inbred C57BL
KW - Molecular Sequence Data
KW - Mycobacterium leprae
KW - Peptides
KW - T-Lymphocytes
AU - Araoz R
AU - Honore N
AU - Banu S
AU - Demangel C
AU - Cissoko Y
AU - Arama C
AU - Uddin MKM
AU - Hadi A
AU - Monot M
AU - Cho S
AU - Ji B
AU - Brennan PJ
AU - Sow S
AU - Cole S
AB - <p>In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-gamma production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.</p>
BT - Microbes and infection
C1 - http://www.ncbi.nlm.nih.gov/pubmed/16797206?dopt=Abstract
DA - 2006 Jul
DO - 10.1016/j.micinf.2006.04.002
IS - 8
J2 - Microbes Infect.
LA - eng
N2 - <p>In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-gamma production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.</p>
PY - 2006
SP - 2270
EP - 6
T2 - Microbes and infection
TI - Towards an immunodiagnostic test for leprosy.
VL - 8
SN - 1286-4579
ER -