TY - JOUR
KW - Adult
KW - Antigens, Bacterial
KW - Cell Adhesion Molecules
KW - Cell Line
KW - Culture Media
KW - Female
KW - HIV Seronegativity
KW - HIV Seropositivity
KW - Humans
KW - Lectins, C-Type
KW - leprosy
KW - Leprosy, Borderline
KW - Leprosy, Tuberculoid
KW - Lipopolysaccharides
KW - Macrophages
KW - Male
KW - Middle Aged
KW - Mycobacterium leprae
KW - Mycobacterium tuberculosis
KW - Receptors, Cell Surface
KW - Th2 Cells
KW - Transfection
AU - Soilleux E J
AU - Sarno E N
AU - Hernandez M O
AU - Moseley E
AU - Horsley J
AU - Lopes U G
AU - Goddard M J
AU - Vowler S L
AU - Coleman N
AU - Shattock R J
AU - Sampaio E P
AB - <p>The clinical spectrum of leprosy is related to patients' immune responses. Non-responsiveness towards Mycobacterium leprae (ML) seems to correlate with a Th2 cytokine profile. The reason for such a polarized immune response remains unclear. The C-type lectin, DC-SIGN, expressed by subsets of dendritic cells (DCs) and macrophages, has previously been associated with Th2 responses. Here we show abundant DC-SIGN expression in lepromatous but not borderline tuberculoid leprosy, in both HIV-positive and HIV-negative patients. Moreover, we demonstrate that DC-SIGN can act as an entry receptor for ML, as it does for M. tuberculosis, through the cell wall component lipoarabinomannan. DC-SIGN is expressed on virtually all ML-containing cells, providing further evidence for its role as a receptor. DC-SIGN may therefore be induced on macrophages in lepromatous leprosy and may then contribute to mycobacterial entry into these cells.</p>
BT - The Journal of pathology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/16583355?dopt=Abstract
DA - 2006 Jun
DO - 10.1002/path.1972
IS - 2
J2 - J. Pathol.
LA - eng
N2 - <p>The clinical spectrum of leprosy is related to patients' immune responses. Non-responsiveness towards Mycobacterium leprae (ML) seems to correlate with a Th2 cytokine profile. The reason for such a polarized immune response remains unclear. The C-type lectin, DC-SIGN, expressed by subsets of dendritic cells (DCs) and macrophages, has previously been associated with Th2 responses. Here we show abundant DC-SIGN expression in lepromatous but not borderline tuberculoid leprosy, in both HIV-positive and HIV-negative patients. Moreover, we demonstrate that DC-SIGN can act as an entry receptor for ML, as it does for M. tuberculosis, through the cell wall component lipoarabinomannan. DC-SIGN is expressed on virtually all ML-containing cells, providing further evidence for its role as a receptor. DC-SIGN may therefore be induced on macrophages in lepromatous leprosy and may then contribute to mycobacterial entry into these cells.</p>
PY - 2006
SP - 182
EP - 9
T2 - The Journal of pathology
TI - DC-SIGN association with the Th2 environment of lepromatous lesions: cause or effect?
VL - 209
SN - 0022-3417
ER -