TY - JOUR
KW - Adult
KW - Antibodies, Anti-Idiotypic
KW - Antigens, Bacterial
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Glycolipids
KW - Humans
KW - Immunoglobulin A
KW - India
KW - leprosy
KW - Male
KW - Middle Aged
KW - Mycobacterium leprae
KW - Predictive Value of Tests
KW - Sensitivity and Specificity
AU - Kumar B
AU - Sinha R
AU - Sehgal S
AB - <p>Purified phenolic glycolipid (PGL-1) from Mycobacterium leprae was used to detect IgG antibodies against PGL-1 in leprosy patients in an enzyme-linked immunosorbent assay (ELISA). A total of 698 sera were screened; they came from patients suffering from leprosy, autoimmune disease, myeloma, tuberculosis and sexually transmitted diseases (STDs). Cases with miscellaneous diseases and persons undergoing AIDS screening were also included. Sera from lepromatous and tuberculoid leprosy patients gave positivity rates of 60.5% and 41.7%, respectively. In non-leprosy cases, the PGL-1 ELISA showed an overall positivity rate of 6.9%; this was greatest in patients with tuberculosis (43.8%) followed by autoimmune diseases (40.9%) and miscellaneous cases including liver diseases (37.9%). This study emphasizes that PGL-1 ELISA has a low predictive value for diagnosis of active infection by Mycobacterium leprae. Positive reactions in a significant percentage of patients with autoimmune disease are intriguing and need indepth study.</p>
BT - The Journal of dermatology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/9609981?dopt=Abstract
DA - 1998 Apr
DO - 10.1111/j.1346-8138.1998.tb02388.x
IS - 4
J2 - J. Dermatol.
LA - eng
N2 - <p>Purified phenolic glycolipid (PGL-1) from Mycobacterium leprae was used to detect IgG antibodies against PGL-1 in leprosy patients in an enzyme-linked immunosorbent assay (ELISA). A total of 698 sera were screened; they came from patients suffering from leprosy, autoimmune disease, myeloma, tuberculosis and sexually transmitted diseases (STDs). Cases with miscellaneous diseases and persons undergoing AIDS screening were also included. Sera from lepromatous and tuberculoid leprosy patients gave positivity rates of 60.5% and 41.7%, respectively. In non-leprosy cases, the PGL-1 ELISA showed an overall positivity rate of 6.9%; this was greatest in patients with tuberculosis (43.8%) followed by autoimmune diseases (40.9%) and miscellaneous cases including liver diseases (37.9%). This study emphasizes that PGL-1 ELISA has a low predictive value for diagnosis of active infection by Mycobacterium leprae. Positive reactions in a significant percentage of patients with autoimmune disease are intriguing and need indepth study.</p>
PY - 1998
SP - 238
EP - 41
T2 - The Journal of dermatology
TI - High incidence of IgG antibodies to phenolic glycolipid in non-leprosy patients in India.
VL - 25
SN - 0385-2407
ER -