TY - JOUR KW - Adult KW - Antibodies, Monoclonal KW - Antigen Presentation KW - Antigens, CD KW - B7-2 Antigen KW - CD4-Positive T-Lymphocytes KW - CD40 Ligand KW - CD8-Positive T-Lymphocytes KW - Dendritic Cells KW - Female KW - HLA Antigens KW - Humans KW - Immunity, Cellular KW - Interferon-gamma KW - Interleukin-2 KW - leprosy KW - Leukocyte Common Antigens KW - Lymphocyte Activation KW - Male KW - Membrane Glycoproteins KW - Membrane Proteins KW - Middle Aged KW - Mycobacterium leprae AU - Makino M AU - Maeda Y AU - Ishii N AB -

We examined the antigenicity of an immunomodulatory protein, major membrane protein (MMP)-II, from Mycobacterium leprae, since host defense against M. leprae largely depends on adaptive immunity. Both unprimed and memory T cells from healthy individuals were stimulated by autologous MMP-II-pulsed monocyte-derived dendritic cells (DCs) to produce IFN-gamma. The DC-mediated IFN-gamma production was dependent on the expression of MHC, CD86, and MMP-II antigens. Memory T cells from paucibacillary (PB) leprosy more extensively responded to MMP-II-pulsed DCs than T cells from healthy individuals, while comparable IFN-gamma was produced by unprimed T cells. Memory T cells from multibacillary leprosy, which are normally believed to be anergic, were activated similarly to those from healthy individuals by MMP-II-pulsed DCs. These results suggest that memory T cells from PB leprosy are primed with MMP-II prior to the manifestation of the disease, and MMP-II is highly antigenic in terms of activation of adaptive immunity.

BT - Cellular immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/15885677?dopt=Abstract DA - 2005 Jan DO - 10.1016/j.cellimm.2005.04.001 IS - 1 J2 - Cell. Immunol. LA - eng N2 -

We examined the antigenicity of an immunomodulatory protein, major membrane protein (MMP)-II, from Mycobacterium leprae, since host defense against M. leprae largely depends on adaptive immunity. Both unprimed and memory T cells from healthy individuals were stimulated by autologous MMP-II-pulsed monocyte-derived dendritic cells (DCs) to produce IFN-gamma. The DC-mediated IFN-gamma production was dependent on the expression of MHC, CD86, and MMP-II antigens. Memory T cells from paucibacillary (PB) leprosy more extensively responded to MMP-II-pulsed DCs than T cells from healthy individuals, while comparable IFN-gamma was produced by unprimed T cells. Memory T cells from multibacillary leprosy, which are normally believed to be anergic, were activated similarly to those from healthy individuals by MMP-II-pulsed DCs. These results suggest that memory T cells from PB leprosy are primed with MMP-II prior to the manifestation of the disease, and MMP-II is highly antigenic in terms of activation of adaptive immunity.

PY - 2005 SP - 53 EP - 60 T2 - Cellular immunology TI - Immunostimulatory activity of major membrane protein-II from Mycobacterium leprae. VL - 233 SN - 0008-8749 ER -