TY - JOUR
KW - Adult
KW - Bacterial Typing Techniques
KW - DNA, Bacterial
KW - Diagnostic Errors
KW - False Positive Reactions
KW - Humans
KW - leprosy
KW - Male
KW - Middle Aged
KW - Mycobacterium avium Complex
KW - Mycobacterium avium-intracellulare Infection
KW - Mycobacterium leprae
KW - Nucleic Acid Amplification Techniques
KW - polymerase chain reaction
KW - RNA, Ribosomal, 16S
KW - Reagent Kits, Diagnostic
KW - Sequence Analysis, DNA
AU - Lefmann M
AU - Moter A
AU - Schweickert B
AU - Göbel U B
AB - <p>Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- positive results. Correct identification of Mycobacterium leprae was possible only by amplification and comparative sequence analysis of the 16S rRNA gene.</p>
BT - Journal of clinical microbiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/15815021?dopt=Abstract
DA - 2005 Apr
DO - 10.1128/JCM.43.4.1928-1929.2005
IS - 4
J2 - J. Clin. Microbiol.
LA - eng
N2 - <p>Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- positive results. Correct identification of Mycobacterium leprae was possible only by amplification and comparative sequence analysis of the 16S rRNA gene.</p>
PY - 2005
SP - 1928
EP - 9
T2 - Journal of clinical microbiology
TI - Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare test.
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081340/pdf/1425-04.pdf
VL - 43
SN - 0095-1137
ER -