TY - JOUR
KW - Amplified Fragment Length Polymorphism Analysis
KW - Animals
KW - Bacterial Proteins
KW - DNA, Bacterial
KW - Emigration and Immigration
KW - Genes, Bacterial
KW - genotype
KW - Humans
KW - India
KW - leprosy
KW - Mycobacterium leprae
KW - Population Dynamics
KW - Sequence Analysis, DNA
KW - Sigma Factor
KW - Skin
KW - Tandem Repeat Sequences
AU - Lavania M
AU - Lal R
AU - Joseph G
AU - Darlong J
AU - Abraham S
AU - Nanda N K
AU - Jadhav R S
AB - <p>Mycobacterium leprae strains from Indian leprosy patients were analyzed using the six base tandem repeat, GACATC, in rpoT gene as genetic marker. DNA was extracted from slit-skin smears and nasal swabs of new untreated as well as treated leprosy patients living in different regions of India. PCR amplification of rpoT gene and sequencing of amplicons showed the presence of two genotype of M. leprae in this study, 73.4% having three copies (ancient Indian type) and 26.6% contain 4 copies (considered to be Japanese and Korean). These genotypes along with other short tandem repeats may help in studying the historical spread of disease and the strains of M. leprae disseminated by various human races that migrated to India from other places of Asia and European countries during our history.</p>
BT - Indian journal of leprosy
C1 - http://www.ncbi.nlm.nih.gov/pubmed/20509339?dopt=Abstract
CN - Infolep Library - available
DA - 2009 Jul-Sep
IS - 3
J2 - Indian J Lepr
LA - eng
N2 - <p>Mycobacterium leprae strains from Indian leprosy patients were analyzed using the six base tandem repeat, GACATC, in rpoT gene as genetic marker. DNA was extracted from slit-skin smears and nasal swabs of new untreated as well as treated leprosy patients living in different regions of India. PCR amplification of rpoT gene and sequencing of amplicons showed the presence of two genotype of M. leprae in this study, 73.4% having three copies (ancient Indian type) and 26.6% contain 4 copies (considered to be Japanese and Korean). These genotypes along with other short tandem repeats may help in studying the historical spread of disease and the strains of M. leprae disseminated by various human races that migrated to India from other places of Asia and European countries during our history.</p>
PY - 2009
SP - 119
EP - 24
T2 - Indian journal of leprosy
TI - Genotypic analysis of Mycobacterium leprae strains from different regions of India on the basis of rpoT.
UR - http://www.ijl.org.in/July-Sept%202009_PDF%20Files/2_Abst%20(119-124).pdf
VL - 81
SN - 0254-9395
ER -