TY - JOUR
KW - Adolescent
KW - Adult
KW - Female
KW - Humans
KW - In Situ Hybridization
KW - leprosy
KW - Male
KW - Middle Aged
KW - Mycobacterium leprae
KW - Skin
AU - Natrajan M
AU - Katoch K
AU - Katoch VM
AU - Sharma V
AU - Singh D
AU - Chauhan DS
AU - Singh H
AB - <p>The present study tests the utility of the in situ hybridization procedure for M. leprae rRNA in the histological diagnosis of early leprosy and clinically suspect leprosy, both diagnostically demanding situations. The histological confirmation obtained with routine histopathology (Haematoxylin-Eosin staining for studying morphologic alterations and Fite-Faraco staining for demonstration of acid-fast bacilli) were 32% for early leprosy and 25% for clinically suspect leprosy. With performance of the in situ hybridization on the histologically unconfirmed cases, the positivity rates obtained were 58.8% and 55%, respectively. The results of the study confirm the utility of the procedure in the diagnostically difficult situations of early and suspect leprosy, and it is proposed that the procedure be employed in situations of clinical doubt.</p>
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
C1 - http://www.ncbi.nlm.nih.gov/pubmed/15485287?dopt=Abstract
DA - 2004 Sep
DO - 10.1489/0020-7349(2004)72<296:ISHITH>2.0.CO;2
IS - 3
J2 - Int. J. Lepr. Other Mycobact. Dis.
LA - eng
N2 - <p>The present study tests the utility of the in situ hybridization procedure for M. leprae rRNA in the histological diagnosis of early leprosy and clinically suspect leprosy, both diagnostically demanding situations. The histological confirmation obtained with routine histopathology (Haematoxylin-Eosin staining for studying morphologic alterations and Fite-Faraco staining for demonstration of acid-fast bacilli) were 32% for early leprosy and 25% for clinically suspect leprosy. With performance of the in situ hybridization on the histologically unconfirmed cases, the positivity rates obtained were 58.8% and 55%, respectively. The results of the study confirm the utility of the procedure in the diagnostically difficult situations of early and suspect leprosy, and it is proposed that the procedure be employed in situations of clinical doubt.</p>
PY - 2004
SP - 296
EP - 305
T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
TI - In situ hybridization in the histological diagnosis of early and clinically suspect leprosy.
UR - http://ila.ilsl.br/pdfs/v72n3a06.pdf
VL - 72
SN - 0148-916X
ER -