TY - JOUR KW - Confidence Intervals KW - Female KW - Gene Frequency KW - Genetic Markers KW - Genetic Predisposition to Disease KW - Haplotypes KW - Humans KW - Interleukin-10 KW - leprosy KW - Logistic Models KW - Male KW - Odds Ratio KW - Polymorphism, Single Nucleotide KW - Promoter Regions, Genetic AU - Moraes M O AU - Pacheco A G AU - Schonkeren J J M AU - Vanderborght P R AU - Nery J A C AU - Santos A R AU - Moraes M E AU - Moraes J R AU - Ottenhoff T H M AU - Sampaio E P AU - Huizinga T W J AU - Sarno E N AB -

We have determined IL-10 promoter genotypes of five single-nucleotide polymorphisms (SNPs): T-3575A, A-2849G, C-2763A, -A-1082G and C-819T. The haplotype frequencies were defined in healthy subjects compared to leprosy patients, and analyzed for their occurrence in multi- (MB) vs paucibacillary (PB) as severe and mild forms of leprosy, respectively. Haplotypes defined by three SNP positions (-3575, -2849 and -2763) captured significant differences between controls and patients (P=0.04). The haplotype carrying -3575A, -2849G and -2763C was associated with resistance to leprosy and to the development of severe forms of the disease using either a binomial (controls vs cases, P=0.005, OR=0.35, CI=0.13-0.91) or ordinal (controls vs PB vs MB, P=0.006, OR=0.32, CI=0.12-0.83) model. By contrast, the IL-10 haplotype -3575T/-2849A/-2763C was found to be associated with susceptibility to leprosy per se (P=0.027, OR=2.37, CI=1.04-5.39), but not leprosy type. The data suggest that the IL-10 locus contributes to the outcome of leprosy.

BT - Genes and immunity C1 - http://www.ncbi.nlm.nih.gov/pubmed/15306847?dopt=Abstract CN - MORAES 2004 DA - 2004 Nov DO - 10.1038/sj.gene.6364122 IS - 7 J2 - Genes Immun. LA - eng N2 -

We have determined IL-10 promoter genotypes of five single-nucleotide polymorphisms (SNPs): T-3575A, A-2849G, C-2763A, -A-1082G and C-819T. The haplotype frequencies were defined in healthy subjects compared to leprosy patients, and analyzed for their occurrence in multi- (MB) vs paucibacillary (PB) as severe and mild forms of leprosy, respectively. Haplotypes defined by three SNP positions (-3575, -2849 and -2763) captured significant differences between controls and patients (P=0.04). The haplotype carrying -3575A, -2849G and -2763C was associated with resistance to leprosy and to the development of severe forms of the disease using either a binomial (controls vs cases, P=0.005, OR=0.35, CI=0.13-0.91) or ordinal (controls vs PB vs MB, P=0.006, OR=0.32, CI=0.12-0.83) model. By contrast, the IL-10 haplotype -3575T/-2849A/-2763C was found to be associated with susceptibility to leprosy per se (P=0.027, OR=2.37, CI=1.04-5.39), but not leprosy type. The data suggest that the IL-10 locus contributes to the outcome of leprosy.

PY - 2004 SP - 592 EP - 5 T2 - Genes and immunity TI - Interleukin-10 promoter single-nucleotide polymorphisms as markers for disease susceptibility and disease severity in leprosy. UR - http://www.nature.com/gene/journal/v5/n7/pdf/6364122a.pdf VL - 5 SN - 1466-4879 ER -