TY - JOUR KW - Amino Acid Motifs KW - Animals KW - Antibodies, Bacterial KW - Antibodies, Monoclonal KW - Antigens, Bacterial KW - Glycolipids KW - Humans KW - leprosy KW - Mice KW - Mice, Inbred BALB C KW - Molecular Mimicry KW - Mycobacterium leprae KW - Peptide Library AU - Youn JH AU - Myung H AU - Liav A AU - Chatterjee D AU - Brennan PJ AU - Choi I AU - Cho S AU - Shin J AB -

Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.

BT - FEMS immunology and medical microbiology C1 - http://www.ncbi.nlm.nih.gov/pubmed/15094167?dopt=Abstract DA - 2004 May 01 DO - 10.1016/j.femsim.2004.01.001 IS - 1 J2 - FEMS Immunol. Med. Microbiol. LA - eng N2 -

Phenolic glycolipid-I (PGL-I), a Mycobacterium leprae-specific antigen, has been widely used for the serodiagnosis of leprosy and has been implicated in the pathogenesis of leprosy. In an effort to produce an alternate antigen of PGL-I, the mimotope peptides of PGL-I, W(T/R)LGPY(V/M), were obtained using a monoclonal antibody, III603.8, specific to PGL-I by a phage library. The biotin-labeled predominant mimotope peptide of PGLP1, WTLGPYV, bound to III603.8 in a dose-dependent manner in an immunoassay. However, PGLP1 did not bind to anti-PGL-I antibodies in the serum samples from leprosy patients that were reactive to PGL-I. Although the mimotope peptide of WTLGPYV was not effective as an alternate antigen of PGL-I for the serodiagnosis of leprosy, but it would be of interest to know how the mimotope peptides mimic the role of PGL-I antigen in the pathogenesis of M. leprae infection.

PY - 2004 SP - 51 EP - 7 T2 - FEMS immunology and medical microbiology TI - Production and characterization of peptide mimotopes of phenolic glycolipid-I of Mycobacterium leprae. VL - 41 SN - 0928-8244 ER -