TY - JOUR
KW - Amino Acid Sequence
KW - Animals
KW - Antigens, Bacterial
KW - Bacterial Proteins
KW - Cross Reactions
KW - Humans
KW - Interferon-gamma
KW - leprosy
KW - Lymphocyte Activation
KW - Molecular Sequence Data
KW - Mycobacterium leprae
KW - Mycobacterium tuberculosis
KW - Sequence Homology
KW - T-Lymphocytes
KW - Tuberculosis
AU - Geluk A
AU - Meijgaarden K E
AU - Franken K L M C
AU - Wieles B
AU - Arend S M
AU - Faber W R
AU - Naafs B
AU - Ottenhoff T H M
AB - <p>Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases.</p>
BT - Scandinavian journal of immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/14723623?dopt=Abstract
DA - 2004 Jan
DO - 10.1111/j.0300-9475.2004.01358.x
IS - 1
J2 - Scand. J. Immunol.
LA - eng
N2 - <p>Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases.</p>
PY - 2004
SP - 66
EP - 70
T2 - Scandinavian journal of immunology
TI - Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis.
VL - 59
SN - 0300-9475
ER -