TY - JOUR
KW - Chromatography, High Pressure Liquid
KW - Clofazimine
KW - Drug Monitoring
KW - Female
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Male
KW - Reproducibility of Results
KW - Sensitivity and Specificity
AU - Queiroz R H C
AU - Pereira R C
AU - Gotardo M A
AU - Cordeiro D S
AU - Melchior E
AB - <p>An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1, v/v) at pH 3.0, after precipitating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25 N sodium acetate buffer at pH 3.0 (74:26, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients.</p>
BT - Journal of analytical toxicology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/14516492?dopt=Abstract
DA - 2003 Sep
DO - 10.1093/jat/27.6.377
IS - 6
J2 - J Anal Toxicol
LA - eng
N2 - <p>An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1, v/v) at pH 3.0, after precipitating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25 N sodium acetate buffer at pH 3.0 (74:26, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients.</p>
PY - 2003
SP - 377
EP - 80
T2 - Journal of analytical toxicology
TI - Determination of clofazimine in leprosy patients by high-performance liquid chromatography.
VL - 27
SN - 0146-4760
ER -