TY - JOUR KW - Adenosine Triphosphate KW - Centrifugation, Density Gradient KW - Humans KW - Leprosy, Borderline KW - Leprosy, lepromatous KW - Mycobacterium leprae KW - Povidone KW - Silicon Dioxide AU - Sharma V K AU - Kaur S AU - Kaur I AU - Thakur M AU - Vaishnavi C AU - Kohli M AU - Ganguly N K AB -

Thirty nine untreated patients of bacilliferous leprosy with a mean bacteriological index of 4.8 and morphological index of 1.3% formed the study group. Adenosine triphosphate assay was carried out by (i) enzyme treatment method in 18 patients and (ii) percoll buoyant density gradient method in 21 patients. ATP content obtained by percoll buoyant density gradient method was significantly higher than that obtained by enzyme treatment method. Percoll buoyant density centrifugation for purification and isolation of bacilli from human leproma is simplier, quicker and can serve as an alternate method of enzyme treatment.

BT - Indian journal of experimental biology C1 - http://www.ncbi.nlm.nih.gov/pubmed/1334044?dopt=Abstract DA - 1992 May IS - 5 J2 - Indian J. Exp. Biol. LA - eng N2 -

Thirty nine untreated patients of bacilliferous leprosy with a mean bacteriological index of 4.8 and morphological index of 1.3% formed the study group. Adenosine triphosphate assay was carried out by (i) enzyme treatment method in 18 patients and (ii) percoll buoyant density gradient method in 21 patients. ATP content obtained by percoll buoyant density gradient method was significantly higher than that obtained by enzyme treatment method. Percoll buoyant density centrifugation for purification and isolation of bacilli from human leproma is simplier, quicker and can serve as an alternate method of enzyme treatment.

PY - 1992 SP - 451 EP - 3 T2 - Indian journal of experimental biology TI - Adenosine triphosphate content of Mycobacterium leprae by percoll buoyant density centrifugation. VL - 30 SN - 0019-5189 ER -