TY - JOUR KW - Adolescent KW - Adult KW - Aged KW - Animals KW - Child KW - Child, Preschool KW - DNA, Protozoan KW - Ethiopia KW - Female KW - Humans KW - Infant KW - Isoenzymes KW - Leishmania KW - Leishmaniasis, Cutaneous KW - Male KW - Middle Aged KW - polymerase chain reaction KW - Polymorphism, Restriction Fragment Length KW - Species Specificity AU - Gadisa E AU - Genetu A AU - Kuru T AU - Jirata D AU - Dagne K AU - Aseffa A AU - Gedamu L AB -

Cutaneous leishmaniasis (CL) is an increasing public health problem in Ethiopia. There is a concern that it is spreading with increased incidence. In this study, we used isoenzyme electrophoresis and internal transcribed spacer one (ITS1) PCR-RFLP techniques to identify Leishmania species from CL patients in Ethiopia. We obtained isolates from 55 localized cutaneous leishmaniasis (LCL), 3 diffused cutaneous leishmaniasis (DCL) and 36 biopsy samples from 34 LCL and 2 DCL cases from All Africa Leprosy and Tuberculosis Rehabilitation and Training Center (ALERT) and clinically diagnosed CL cases from Ochollo village. Both isoenzyme and ITS1 PCR-RFLP techniques showed that Leishmania aethiopica (L. aethiopica) was the aetiologic agent in all cases. Our study also showed that ITS1 PCR-RFLP could identify Leishmania species from biopsy samples and suggests the method could be used for epidemiological surveillance of leishmaniasis in Ethiopia and for species-specific diagnosis.

BT - Experimental parasitology C1 - http://www.ncbi.nlm.nih.gov/pubmed/17083934?dopt=Abstract DA - 2007 Apr DO - 10.1016/j.exppara.2006.09.014 IS - 4 J2 - Exp. Parasitol. LA - eng N2 -

Cutaneous leishmaniasis (CL) is an increasing public health problem in Ethiopia. There is a concern that it is spreading with increased incidence. In this study, we used isoenzyme electrophoresis and internal transcribed spacer one (ITS1) PCR-RFLP techniques to identify Leishmania species from CL patients in Ethiopia. We obtained isolates from 55 localized cutaneous leishmaniasis (LCL), 3 diffused cutaneous leishmaniasis (DCL) and 36 biopsy samples from 34 LCL and 2 DCL cases from All Africa Leprosy and Tuberculosis Rehabilitation and Training Center (ALERT) and clinically diagnosed CL cases from Ochollo village. Both isoenzyme and ITS1 PCR-RFLP techniques showed that Leishmania aethiopica (L. aethiopica) was the aetiologic agent in all cases. Our study also showed that ITS1 PCR-RFLP could identify Leishmania species from biopsy samples and suggests the method could be used for epidemiological surveillance of leishmaniasis in Ethiopia and for species-specific diagnosis.

PY - 2007 SP - 339 EP - 43 T2 - Experimental parasitology TI - Leishmania (Kinetoplastida): species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia. VL - 115 SN - 0014-4894 ER -