TY - JOUR
KW - Acetylglucosaminidase
KW - Adult
KW - Glucuronidase
KW - Humans
KW - leprosy
KW - Middle Aged
KW - Monocytes
KW - Zymosan
AU - Kumar B
AU - Jaswal S
AU - Vaishnavi C
AU - Thakur M
AU - Kaur S
AU - Ganguly N K
AB - <p>Release of acid hydrolases by blood monocytes (BM) of leprosy patients both before and after 6 months of chemotherapy was measured fluorimetrically. Monocyte cultures were set up for spontaneous as well as zymosan dependent enzyme release measured after 2 hrs and 24 hrs of culture. In the untreated multibacillary group (BL/LL) a significantly higher (P < 0.001) release of both B-glucuronidase (BG) and N-acetyl glucosaminidase (NAG) was observed compared to the paucibacillary group (BT/TT) and healthy controls. On comparing the BT/TT group with controls a significant decrease (P < 0.001) in zymosan dependent NAG release was observed in the former group at 2 hrs culture. After 6 months of antileprosy therapy, a significant decrease (P < 0.05) in BG release was observed from BM of multibacillary patients, whereas NAG activity increased significantly (P < 0.05) in the paucibacillary group compared to the controls. The results of the present study suggest that non-oxidative metabolic status of BM vary within the leprosy spectrum.</p>
BT - Journal of hygiene, epidemiology, microbiology, and immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/1300357?dopt=Abstract
DA - 1992
IS - 4
J2 - J Hyg Epidemiol Microbiol Immunol
LA - eng
N2 - <p>Release of acid hydrolases by blood monocytes (BM) of leprosy patients both before and after 6 months of chemotherapy was measured fluorimetrically. Monocyte cultures were set up for spontaneous as well as zymosan dependent enzyme release measured after 2 hrs and 24 hrs of culture. In the untreated multibacillary group (BL/LL) a significantly higher (P < 0.001) release of both B-glucuronidase (BG) and N-acetyl glucosaminidase (NAG) was observed compared to the paucibacillary group (BT/TT) and healthy controls. On comparing the BT/TT group with controls a significant decrease (P < 0.001) in zymosan dependent NAG release was observed in the former group at 2 hrs culture. After 6 months of antileprosy therapy, a significant decrease (P < 0.05) in BG release was observed from BM of multibacillary patients, whereas NAG activity increased significantly (P < 0.05) in the paucibacillary group compared to the controls. The results of the present study suggest that non-oxidative metabolic status of BM vary within the leprosy spectrum.</p>
PY - 1992
SP - 405
EP - 11
T2 - Journal of hygiene, epidemiology, microbiology, and immunology
TI - Release of acid hydrolases in spectrum of human leprosy.
VL - 36
SN - 0022-1732
ER -