TY - JOUR
KW - Animals
KW - Antigens, Protozoan
KW - Concanavalin A
KW - Electrophoresis, Polyacrylamide Gel
KW - Epitopes
KW - Humans
KW - Leishmania donovani
KW - Leishmaniasis, Visceral
KW - Serologic Tests
AU - Bhattacharya M
AU - Banerjee N
AU - Ghosh D K
AB - <p>A glycoconjugate antigen of 27-39 kDa was isolated from a cell-free extract of Leishmania donovani by affinity chromatography using a Concanavalin-A sepharose-4B column and eluted with 0.5 M alpha-methylmannoside. The antigen was recognized specifically by sera from kala-azar (visceral leishmaniasis) patients and did not react with sera from tuberculosis, leprosy or malaria patients. The antigen may therefore be useful in developing a serodiagnostic assay for visceral leishmaniasis.</p>
BT - Annals of tropical medicine and parasitology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/1281399?dopt=Abstract
DA - 1992 Aug
DO - 10.1080/00034983.1992.11812676
IS - 4
J2 - Ann Trop Med Parasitol
LA - eng
N2 - <p>A glycoconjugate antigen of 27-39 kDa was isolated from a cell-free extract of Leishmania donovani by affinity chromatography using a Concanavalin-A sepharose-4B column and eluted with 0.5 M alpha-methylmannoside. The antigen was recognized specifically by sera from kala-azar (visceral leishmaniasis) patients and did not react with sera from tuberculosis, leprosy or malaria patients. The antigen may therefore be useful in developing a serodiagnostic assay for visceral leishmaniasis.</p>
PY - 1992
SP - 341
EP - 5
T2 - Annals of tropical medicine and parasitology
TI - Leishmania donovani: isolation of a concanavalin-A specific antigen and its evaluation for serodiagnosis of visceral leishmaniasis.
VL - 86
SN - 0003-4983
ER -