TY - JOUR KW - Animals KW - Antibodies KW - Chorionic Gonadotropin KW - Clinical Trials, Phase II as Topic KW - Contraceptive Agents, Female KW - Female KW - Humans KW - Immunologic Factors KW - Mice KW - Mice, Inbred C3H KW - Mycobacterium KW - Vaccines, Synthetic AU - Purswani S AU - Talwar G P AU - Vohra R AU - Pal R AU - Panda A AU - Lohiya NK AU - Gupta JC AB -

The objective of this work was to identify a human use-permissible adjuvant to enhance significantly the antibody response to a recombinant anti-hCG vaccine. Previous Phase II efficacy trials in sexually active women have demonstrated the prevention of pregnancy at hCG bioneutralization titers of 50ng/ml or more. Mycobacterium indicus pranii (MIP), a non-pathogenic Mycobacterium employed as an autoclaved suspension in aqueous buffer, significantly increased antibody titers in the FVB strain of mice. Three other genetic strains of mice: SJL, C3H, and C57Bl/6 responded with antibody titers several-fold higher than 50 ng/ml, which is the protective threshold in women, although there were differences in the peak titers attained. In addition, the duration of the antibody response was lengthened. The vaccine hCGβ-LTB, given together with MIP, induces both a Th1 and Th2 response, which is reflected in the production of not only IgG1, but also a high proportion of IgG2a and IgG2b antibodies.

BT - Journal of reproductive immunology C1 -

http://www.ncbi.nlm.nih.gov/pubmed/21885129?dopt=Abstract

DO - 10.1016/j.jri.2011.06.099 IS - 1-2 J2 - J. Reprod. Immunol. LA - eng N2 -

The objective of this work was to identify a human use-permissible adjuvant to enhance significantly the antibody response to a recombinant anti-hCG vaccine. Previous Phase II efficacy trials in sexually active women have demonstrated the prevention of pregnancy at hCG bioneutralization titers of 50ng/ml or more. Mycobacterium indicus pranii (MIP), a non-pathogenic Mycobacterium employed as an autoclaved suspension in aqueous buffer, significantly increased antibody titers in the FVB strain of mice. Three other genetic strains of mice: SJL, C3H, and C57Bl/6 responded with antibody titers several-fold higher than 50 ng/ml, which is the protective threshold in women, although there were differences in the peak titers attained. In addition, the duration of the antibody response was lengthened. The vaccine hCGβ-LTB, given together with MIP, induces both a Th1 and Th2 response, which is reflected in the production of not only IgG1, but also a high proportion of IgG2a and IgG2b antibodies.

PY - 2011 SP - 24 EP - 30 T2 - Journal of reproductive immunology TI - Mycobacterium indicus pranii is a potent immunomodulator for a recombinant vaccine against human chorionic gonadotropin. VL - 91 SN - 1872-7603 ER -