TY - JOUR
KW - Vaccines, Synthetic
KW - Vaccines, Contraceptive
KW - Vaccination
KW - Recombinant Fusion Proteins
KW - Pregnancy
KW - Mice, Inbred BALB C
KW - Mice
KW - Humans
KW - Female
KW - Escherichia coli Proteins
KW - Enterotoxins
KW - DNA
KW - Contraception, Immunologic
KW - Chorionic Gonadotropin, beta Subunit, Human
KW - Bacterial Toxins
KW - Antibody Formation
KW - Antibodies
KW - Animals
AU - Nand KN
AU - Gupta JC
AU - Panda A
AU - Jain S
AU - Talwar G
AB - <p><strong>PROBLEM: </strong>Necessity to elicit antibody response above the protective threshold titres by sexually active women immunized to prevent pregnancy.</p>

<p><strong>METHOD OF STUDY: </strong>Recombinant hCGβ-LTB vaccine expressed as both DNA and protein. Balb C mice employed for testing immunogenicity.</p>

<p><strong>RESULTS: </strong>Necessity to give three primary injections of the vaccine to elicit proper antibody response. Immunization twice with DNA form of the vaccine at fortnightly interval followed by the protein elicits a distinctly higher antibody response than proteinic vaccine alone. Antibodies generated are bio-effective against hCG.</p>

<p><strong>CONCLUSION: </strong>Immunization with the DNA form of the recombinant hCGβ-LTB vaccine twice at fortnightly interval followed by the proteinic form of the vaccine induces distinctly higher antibody response.</p>

BT - American journal of reproductive immunology (New York, N.Y. : 1989)
C1 - <p>http://www.ncbi.nlm.nih.gov/pubmed/25917014?dopt=Abstract</p>

DO - 10.1111/aji.12388
IS - 4
J2 - Am. J. Reprod. Immunol.
LA - eng
N2 - <p><strong>PROBLEM: </strong>Necessity to elicit antibody response above the protective threshold titres by sexually active women immunized to prevent pregnancy.</p>

<p><strong>METHOD OF STUDY: </strong>Recombinant hCGβ-LTB vaccine expressed as both DNA and protein. Balb C mice employed for testing immunogenicity.</p>

<p><strong>RESULTS: </strong>Necessity to give three primary injections of the vaccine to elicit proper antibody response. Immunization twice with DNA form of the vaccine at fortnightly interval followed by the protein elicits a distinctly higher antibody response than proteinic vaccine alone. Antibodies generated are bio-effective against hCG.</p>

<p><strong>CONCLUSION: </strong>Immunization with the DNA form of the recombinant hCGβ-LTB vaccine twice at fortnightly interval followed by the proteinic form of the vaccine induces distinctly higher antibody response.</p>

PY - 2015
SP - 302
EP - 8
T2 - American journal of reproductive immunology (New York, N.Y. : 1989)
TI - Priming with DNA Enhances Considerably the Immunogenicity of hCG β-LTB Vaccine.
VL - 74
SN - 1600-0897
ER -