TY - JOUR
AU - Wowk PF
AU - Franco LH
AU - Fonseca DM
AU - Paula MO
AU - Vianna ÉDSO
AU - Wendling AP
AU - Augusto VM
AU - Elói-Santos SM
AU - Teixeira-Carvalho A
AU - Silva FDC
AU - Vinhas SA
AU - Martins-Filho OA
AU - Palaci M
AU - Silva CL
AU - Bonato VLD
AB - <p>Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4(+) or CD8(+) cell were assessed by flow cytometry. The CD4(+) cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8(+) cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN-gamma(+) cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.</p>

BT - Human vaccines & immunotherapeutics
C1 - <p>http://www.ncbi.nlm.nih.gov/pubmed/28059670?dopt=Abstract</p>

DO - 10.1080/21645515.2016.1264547
J2 - Hum Vaccin Immunother
LA - eng
N2 - <p>Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4(+) or CD8(+) cell were assessed by flow cytometry. The CD4(+) cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8(+) cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN-gamma(+) cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.</p>

PY - 2017
EP - 0
T2 - Human vaccines & immunotherapeutics
TI - Mycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared to tuberculosis patients.
SN - 2164-554X
ER -