TY - JOUR
KW - Antibodies, Bacterial
KW - Antibodies, Monoclonal
KW - Antigens, Bacterial
KW - Binding, Competitive
KW - Enzyme-Linked Immunosorbent Assay
KW - Glycolipids
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Leprosy, lepromatous
KW - Leprosy, Tuberculoid
KW - Mycobacterium leprae
KW - Predictive Value of Tests
KW - Sensitivity and Specificity
AU - Parkash O
AU - Girdhar B K
AB - <p>The performances of two Mycobacterium leprae specific serological assays namely: phenolicglycolipid-l-based enzyme-linked immunosorbent assay (PGL-ELISA) and a monoclonal antibody-based inhibition test (MAIT) were studied for there efficiency to detect M. leprae infection. As usual, both the tests were more sensitive to detect lepromatous leprosy patients than tuberculoid type of leprosy patients. Considering the efficiency to detect leprosy patients, the MAIT was slightly more sensitive and specific than PGL-ELISA. When the results of both assays are considered together, a better sensitivity (over the sensitivity of individual assay) was obtained while maintaining good enough specificity. These findings point out that a combinatorial approach for detection of M. leprae infection would be a better strategy to detect M. leprae infection. Hence, it may act as a better tool for measurement of bacterial load in the patients.</p>
BT - Acta leprologica
C1 - http://www.ncbi.nlm.nih.gov/pubmed/12136738?dopt=Abstract
CN - PARKASH 2001
DA - 2001-2002
IS - 2
J2 - Acta Leprol
LA - eng
N2 - <p>The performances of two Mycobacterium leprae specific serological assays namely: phenolicglycolipid-l-based enzyme-linked immunosorbent assay (PGL-ELISA) and a monoclonal antibody-based inhibition test (MAIT) were studied for there efficiency to detect M. leprae infection. As usual, both the tests were more sensitive to detect lepromatous leprosy patients than tuberculoid type of leprosy patients. Considering the efficiency to detect leprosy patients, the MAIT was slightly more sensitive and specific than PGL-ELISA. When the results of both assays are considered together, a better sensitivity (over the sensitivity of individual assay) was obtained while maintaining good enough specificity. These findings point out that a combinatorial approach for detection of M. leprae infection would be a better strategy to detect M. leprae infection. Hence, it may act as a better tool for measurement of bacterial load in the patients.</p>
PY - 2001
SP - 63
EP - 9
T2 - Acta leprologica
TI - A comparative and combinatorial study on two serological assays for detection of Mycobacterium leprae infection.
VL - 12
SN - 0001-5938
ER -