TY - JOUR
KW - Animals
KW - CD4-Positive T-Lymphocytes
KW - CD8-Positive T-Lymphocytes
KW - Cytokines
KW - Disease Models, Animal
KW - Flow Cytometry
KW - Foot
KW - Gene Deletion
KW - Humans
KW - Immunohistochemistry
KW - Interferon-gamma
KW - leprosy
KW - Lymph Nodes
KW - Lymphocyte Activation
KW - Macrophages, Peritoneal
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Knockout
KW - Mycobacterium leprae
AU - Adams LW
AU - Scollard D
AU - Ray NA
AU - Cooper AM
AU - Frank A
AU - Orme I
AU - Krahenbuhl JL
AB - <p>Mycobacterium leprae infection was evaluated in interferon-gamma knockout (GKO) mice. At 4 months, growth of the bacilli in the footpads of GKO mice plateaued a log(10) higher than that in control mice. Control mice exhibited mild lymphocytic and histiocytic infiltrates, whereas GKO mice developed large, unorganized infiltrates of epithelioid macrophages and scattered CD4 and CD8 T cells. Flow cytometric analysis of popliteal lymph node cells demonstrated similar profiles of T cells; however, GKO cells exhibited an elevated proliferative response to M. leprae antigen. Expression of inducible nitric oxide synthase mRNA was decreased in GKO mice, whereas macrophage inflammatory protein-1alpha and interleukin-4 and -10 mRNA expression were augmented. Control and GKO activated macrophages inhibited bacterial metabolism and produced nitrite. Thus, although deficient in an important Th1 cytokine, GKO mice possess compensatory mechanisms to control M. leprae growth and feature elements resembling mid-borderline leprosy in humans.</p>
BT - The Journal of infectious diseases
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11865434?dopt=Abstract
DA - 2002 Feb 15
DO - 10.1086/338002
J2 - J. Infect. Dis.
LA - eng
N2 - <p>Mycobacterium leprae infection was evaluated in interferon-gamma knockout (GKO) mice. At 4 months, growth of the bacilli in the footpads of GKO mice plateaued a log(10) higher than that in control mice. Control mice exhibited mild lymphocytic and histiocytic infiltrates, whereas GKO mice developed large, unorganized infiltrates of epithelioid macrophages and scattered CD4 and CD8 T cells. Flow cytometric analysis of popliteal lymph node cells demonstrated similar profiles of T cells; however, GKO cells exhibited an elevated proliferative response to M. leprae antigen. Expression of inducible nitric oxide synthase mRNA was decreased in GKO mice, whereas macrophage inflammatory protein-1alpha and interleukin-4 and -10 mRNA expression were augmented. Control and GKO activated macrophages inhibited bacterial metabolism and produced nitrite. Thus, although deficient in an important Th1 cytokine, GKO mice possess compensatory mechanisms to control M. leprae growth and feature elements resembling mid-borderline leprosy in humans.</p>
PY - 2002
SP - S1
EP - 8
T2 - The Journal of infectious diseases
TI - The study of Mycobacterium leprae infection in interferon-gamma gene--disrupted mice as a model to explore the immunopathologic spectrum of leprosy.
VL - 185 Suppl 1
SN - 0022-1899
ER -