TY - JOUR
KW - Animals
KW - Antigens, Bacterial
KW - Bacterial Proteins
KW - Biopsy
KW - Densitometry
KW - DNA, Bacterial
KW - Drug Therapy, Combination
KW - Electrophoresis, Agar Gel
KW - Glycolipids
KW - Humans
KW - Immunoglobulin M
KW - Leprostatic Agents
KW - leprosy
KW - Longitudinal studies
KW - Mice
KW - Mice, Nude
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Reproducibility of Results
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - RNA, Messenger
AU - Chae G
AU - Kim M
AU - Kang T
AU - Lee S
AU - Shin H
AU - Kim J
AU - Ko Y
AU - Kim S
AU - Kim N
AB - <p>DNA-PCR and reverse transcription (RT)-PCR for the 18-kDa protein of Mycobacterium leprae were used to examine the efficacy of multi-drug therapy (MDT) in leprosy. MDT was administered for 0-24 months. Fourteen (63.6%) of 22 patients showed positive PCR results after treatment for 12 months and the positive results decreased to 30% after 24 months of MDT. These results did not correlate with the bacterial index (BI) or the IgM antibody titre for the phenolic glycolipid (PGL)-1. One-dimensional densitometric analysis of agarose gels from PCR from the longitudinal study showed a gradual reduction of the 360-bp band after 12-24 months of MDT. RT-PCR for mRNA of the 18-kDa protein successfully tracked bacterial RNA changes in the biopsies and confirmed a decrease in the RNA of M. leprae in patients after MDT for 12 months. Thus, DNA- and RT-PCR for the 18-kDa protein of M. leprae are effective in assessing the efficacy of MDT for leprosy.</p>
BT - Journal of medical microbiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11990494?dopt=Abstract
DA - 2002 May
DO - 10.1099/0022-1317-51-5-417
IS - 5
J2 - J. Med. Microbiol.
LA - eng
N2 - <p>DNA-PCR and reverse transcription (RT)-PCR for the 18-kDa protein of Mycobacterium leprae were used to examine the efficacy of multi-drug therapy (MDT) in leprosy. MDT was administered for 0-24 months. Fourteen (63.6%) of 22 patients showed positive PCR results after treatment for 12 months and the positive results decreased to 30% after 24 months of MDT. These results did not correlate with the bacterial index (BI) or the IgM antibody titre for the phenolic glycolipid (PGL)-1. One-dimensional densitometric analysis of agarose gels from PCR from the longitudinal study showed a gradual reduction of the 360-bp band after 12-24 months of MDT. RT-PCR for mRNA of the 18-kDa protein successfully tracked bacterial RNA changes in the biopsies and confirmed a decrease in the RNA of M. leprae in patients after MDT for 12 months. Thus, DNA- and RT-PCR for the 18-kDa protein of M. leprae are effective in assessing the efficacy of MDT for leprosy.</p>
PY - 2002
SP - 417
EP - 22
T2 - Journal of medical microbiology
TI - DNA-PCR and RT-PCR for the 18-kDa gene of Mycobacterium leprae to assess the efficacy of multi-drug therapy for leprosy.
VL - 51
SN - 0022-2615
ER -