TY - JOUR
KW - Adult
KW - Aged
KW - Antibiotics, Antitubercular
KW - Disease Models, Animal
KW - DNA gyrase
KW - DNA, Bacterial
KW - DNA-Directed RNA Polymerases
KW - Drug Resistance, Bacterial
KW - Humans
KW - leprosy
KW - Microbial Sensitivity Tests
KW - Middle Aged
KW - Mutation
KW - Mycobacterium leprae
KW - Ofloxacin
KW - Plant Proteins
KW - Recurrence
KW - Rifampin
AU - Cambau E
AU - Bonnafous P
AU - Perani E
AU - Sougakoff W
AU - Ji B
AU - Jarlier V
AB - <p>Molecular detection of rifampin resistance (rpoB analysis) in Mycobacterium leprae was determined for 49 patients who experienced relapse of multibacillary leprosy and for 34 untreated patients. Molecular detection of ofloxacin resistance (gyrA analysis) was determined for the 12 patients who experienced relapse and who had received ofloxacin. Results of molecular tests were compared with the reference susceptibility test in the mouse footpad. Overall, the efficiency of molecular detection--that is, positive DNA amplification--was 95%, whereas that of the in vivo test was 55% (P<.001). Results of molecular detection and in vivo test were fully concordant when both were available--that is, for 35 rifampin--sensitive cases of leprosy (no rpoB mutation), 4 ofloxacin-sensitive cases (no gyrA mutation), 11 rifampin-resistant cases (rpoB missense mutations), and 1 ofloxacin-resistant case (gyrA mutation). rpoB and gyrA analysis appears to be an effective method for detection of rifampin and ofloxacin resistance in patients with leprosy.</p>
BT - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11731943?dopt=Abstract
DA - 2002 Jan 01
DO - 10.1086/324623
IS - 1
J2 - Clin. Infect. Dis.
LA - eng
N2 - <p>Molecular detection of rifampin resistance (rpoB analysis) in Mycobacterium leprae was determined for 49 patients who experienced relapse of multibacillary leprosy and for 34 untreated patients. Molecular detection of ofloxacin resistance (gyrA analysis) was determined for the 12 patients who experienced relapse and who had received ofloxacin. Results of molecular tests were compared with the reference susceptibility test in the mouse footpad. Overall, the efficiency of molecular detection--that is, positive DNA amplification--was 95%, whereas that of the in vivo test was 55% (P<.001). Results of molecular detection and in vivo test were fully concordant when both were available--that is, for 35 rifampin--sensitive cases of leprosy (no rpoB mutation), 4 ofloxacin-sensitive cases (no gyrA mutation), 11 rifampin-resistant cases (rpoB missense mutations), and 1 ofloxacin-resistant case (gyrA mutation). rpoB and gyrA analysis appears to be an effective method for detection of rifampin and ofloxacin resistance in patients with leprosy.</p>
PY - 2002
SP - 39
EP - 45
T2 - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
TI - Molecular detection of rifampin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy.
UR - http://cid.oxfordjournals.org/content/34/1/39.full.pdf+html
VL - 34
SN - 1537-6591
ER -