A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
BT - Leprosy review C1 - http://www.ncbi.nlm.nih.gov/pubmed/11826480?dopt=Abstract CN - Infolep Library - available DA - 2001 Dec DO - 10.5935/0305-7518.20010052 IS - 4 J2 - Lepr Rev LA - eng N2 -A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
PY - 2001 SP - 441 EP - 8 T2 - Leprosy review TI - A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae. UR - http://leprev.ilsl.br/pdfs/2001/v72n4/pdf/v72n4a07.pdf VL - 72 SN - 0305-7518 ER -