TY - JOUR KW - Amino Acid Sequence KW - Bacterial Proteins KW - HLA-DR Antigens KW - Humans KW - Interferon-gamma KW - Interleukin-5 KW - leprosy KW - Molecular Sequence Data KW - Mycobacterium leprae KW - T-Lymphocytes AU - Ohyama H AU - Matsushita S AU - Nishimura F AU - Kato N AU - Hatano K AU - Takashiba S AU - Murayama Y AB -

Major membrane protein II (MMP II) of Mycobacterium leprae (M. leprae) is a 22kDa protein inducing humoral immune response in leprosy patients. MMP II-specific bulk T cell lines were established from leprosy patients to determine major T cell epitopes in MMP II and to evaluate lymphokine production induced by MMP II. These bulk T cell lines reacted to one or more peptides in the locus of amino acid residues from 23 to 109 of MMP II. The proliferative responses of all T cell lines were mainly inhibited by the addition of anti-DRB1 mAb. Many bulk T cell lines induced IFN-gamma, IL-5, but not IL-4. However, it was not possible to distinguish the LL or TT types of leprosy based on the pattern of T cell epitopes and the lymphokine productivity in the responses against MMP II. Thus, it appears that T cell response to MMP II is restricted by the HLA-DRB1 molecule, but not by DQ and DP molecules, which results in the induction of IFN-gamma production.

BT - Vaccine C1 - http://www.ncbi.nlm.nih.gov/pubmed/11672912?dopt=Abstract DA - 2001 Nov 12 DO - 10.1016/s0264-410x(01)00354-1 IS - 3-4 J2 - Vaccine LA - eng N2 -

Major membrane protein II (MMP II) of Mycobacterium leprae (M. leprae) is a 22kDa protein inducing humoral immune response in leprosy patients. MMP II-specific bulk T cell lines were established from leprosy patients to determine major T cell epitopes in MMP II and to evaluate lymphokine production induced by MMP II. These bulk T cell lines reacted to one or more peptides in the locus of amino acid residues from 23 to 109 of MMP II. The proliferative responses of all T cell lines were mainly inhibited by the addition of anti-DRB1 mAb. Many bulk T cell lines induced IFN-gamma, IL-5, but not IL-4. However, it was not possible to distinguish the LL or TT types of leprosy based on the pattern of T cell epitopes and the lymphokine productivity in the responses against MMP II. Thus, it appears that T cell response to MMP II is restricted by the HLA-DRB1 molecule, but not by DQ and DP molecules, which results in the induction of IFN-gamma production.

PY - 2001 SP - 475 EP - 82 T2 - Vaccine TI - T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy. VL - 20 SN - 0264-410X ER -