TY - JOUR KW - leprosy KW - Mycobacterium leprae KW - GentleMACS dissociator KW - mouse footpad tissue KW - purification AU - Williams DL AU - Adams LW AU - Lahiri R AB -
Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FP). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.
BT - Journal of microbiological methods C1 - http://www.ncbi.nlm.nih.gov/pubmed/25019518?dopt=Abstract DO - 10.1016/j.mimet.2014.06.019 J2 - J. Microbiol. Methods LA - eng N2 -Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FP). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.
PY - 2014 T2 - Journal of microbiological methods TI - Semi-automated Protocol for Purification of Mycobacterium leprae From Tissues Using the gentleMACS™ Octo Dissociator. SN - 1872-8359 ER -