TY - JOUR
KW - leprosy
KW - Mycobacterium leprae
KW - GentleMACS dissociator
KW - mouse footpad tissue
KW - purification
AU - Williams DL
AU - Adams LW
AU - Lahiri R
AB - <p>Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FP). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.</p>
BT - Journal of microbiological methods
C1 - http://www.ncbi.nlm.nih.gov/pubmed/25019518?dopt=Abstract
DO - 10.1016/j.mimet.2014.06.019
J2 - J. Microbiol. Methods
LA - eng
N2 - <p>Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FP). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.</p>
PY - 2014
T2 - Journal of microbiological methods
TI - Semi-automated Protocol for Purification of Mycobacterium leprae From Tissues Using the gentleMACS™ Octo Dissociator.
SN - 1872-8359
ER -