TY - JOUR
KW - Activities of Daily Living
KW - Aged
KW - Brazil
KW - Cross-Sectional Studies
KW - Female
KW - Health Status Indicators
KW - Home Care Services
KW - Humans
KW - leprosy
KW - Linear Models
KW - Male
KW - Mental Health
KW - Perception
KW - Psychiatric Status Rating Scales
KW - Quality of Life
KW - Reproducibility of Results
KW - Socioeconomic Factors
KW - Surveys and Questionnaires
KW - World Health Organization
AU - Prapanna P
AU - Srivastava R
AU - Arora VK
AU - Singh N
AU - Bhatia A
AU - Kaur I
AB - The aim of the study is to determine whether immunostaining for mycobacterial antigen can contribute to the cytological diagnosis of extrapulmonary tuberculosis (EPTB). The study was carried out on aspirated material of lymph nodes, and other accessible sites, from 65 patients with clinical diagnosis of tuberculosis (TB). Twenty patients, diagnosed by fine-needle aspiration, with non-tuberculous granulomas served as controls. The diagnosis of TB was based on the demonstration of acid-fast bacilli (AFB), culture positivity for Mycobacterium tuberculosis (M. tuberculosis), or response to treatment with standard anti-tubercular therapy. Immunostaining was done using polyclonal antibody to mycobacteria. AFB positivity by Ziehl Neelsen (ZN) staining was 21%, 65.38%, and 68% respectively in Pattern 1 (granulomas alone), in Pattern 2 (granulomas with necrosis), and in Pattern 3 (necrosis alone). Overall AFB positivity was 56.92%. Twenty-eight of 65 cases were negative for AFB on direct smear. Culture was positive in 46% (13/28). Sensitivity and specificity of immunostaining were 96.92% (63/65) and 95%, respectively. Immunoreactivity was seen in 26 (92.8%) of 28 cases which were negative by ZN staining. Except in the case of leprosy, in which cross reactivity was seen, there was no immunoreactivity in the control group. Immunocytochemistry (ICC) had high sensitivity (96.2%) and specificity (95%) in the diagnosis of EPTB. ICC may be a useful adjunct to evaluation of cytomorphology and ZN staining. Diagn. Cytopathol 2014;42:391-395. © 2013 Wiley Periodicals, Inc.
BT - Diagnostic cytopathology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/24166859?dopt=Abstract
DA - 2014 May
DO - 10.1007/s11136-013-0590-7
IS - 5
J2 - Diagn. Cytopathol.
LA - eng
N2 - The aim of the study is to determine whether immunostaining for mycobacterial antigen can contribute to the cytological diagnosis of extrapulmonary tuberculosis (EPTB). The study was carried out on aspirated material of lymph nodes, and other accessible sites, from 65 patients with clinical diagnosis of tuberculosis (TB). Twenty patients, diagnosed by fine-needle aspiration, with non-tuberculous granulomas served as controls. The diagnosis of TB was based on the demonstration of acid-fast bacilli (AFB), culture positivity for Mycobacterium tuberculosis (M. tuberculosis), or response to treatment with standard anti-tubercular therapy. Immunostaining was done using polyclonal antibody to mycobacteria. AFB positivity by Ziehl Neelsen (ZN) staining was 21%, 65.38%, and 68% respectively in Pattern 1 (granulomas alone), in Pattern 2 (granulomas with necrosis), and in Pattern 3 (necrosis alone). Overall AFB positivity was 56.92%. Twenty-eight of 65 cases were negative for AFB on direct smear. Culture was positive in 46% (13/28). Sensitivity and specificity of immunostaining were 96.92% (63/65) and 95%, respectively. Immunoreactivity was seen in 26 (92.8%) of 28 cases which were negative by ZN staining. Except in the case of leprosy, in which cross reactivity was seen, there was no immunoreactivity in the control group. Immunocytochemistry (ICC) had high sensitivity (96.2%) and specificity (95%) in the diagnosis of EPTB. ICC may be a useful adjunct to evaluation of cytomorphology and ZN staining. Diagn. Cytopathol 2014;42:391-395. © 2013 Wiley Periodicals, Inc.
PY - 2014
SP - 391
EP - 5
T2 - Diagnostic cytopathology
TI - Immunocytochemical detection of mycobacterial antigen in extrapulmonary tuberculosis.
VL - 42
SN - 1097-0339
ER -