TY - JOUR KW - Animals KW - Bacterial Proteins KW - Biotechnology KW - Cell Proliferation KW - Chaperonin 60 KW - Cytokines KW - Fungal Vaccines KW - Gene Transfer Techniques KW - Immunity, Humoral KW - Immunoglobulin G KW - Lactic Acid KW - Liposomes KW - Lung KW - Male KW - Mice KW - Mice, Inbred BALB C KW - Mycobacterium leprae KW - Nanotechnology KW - Nitric Oxide KW - Paracoccidioides KW - Paracoccidioidomycosis KW - Polyglycolic Acid KW - Polylactic Acid-Polyglycolic Acid Copolymer KW - Spleen KW - Vaccines, DNA AU - Ribeiro A M AU - Souza A C O AU - Amaral A C AU - Vasconcelos N M AU - Jeronimo M S AU - Carneiro F P AU - Faccioli L H AU - Felipe M S S AU - Silva C L AU - Bocca A L AB -

Vaccines play an essential role in keeping humans healthy. Innovative approaches to their use include the utilization of plasmid DNA encoding sequences to express foreign antigens. DNAhsp65 from Mycobacterium leprae is suitable for this purpose due to its ability to elicit a powerful immune response. Controlled release systems represent a promising approach to delivering vaccines. In this work, we used liposomes or PLGA systems to deliver DNAhsp65 to treat the pulmonary fungal infection Paracoccidioidomycosis. Both formulations modulated a protective immune response and reduced the pulmonary fungal burden even in the groups receiving less than four times the amount of the DNAhps65 entrapped within the nanoparticles. Although both systems had the same effective therapeutic results, the advantage of the liposome formulation was that it was administered intranasally, which may be more easily accepted by patients. These systems are a great alternative to be considered as adjuvant vaccine therapy for systemic mycosis.

BT - Journal of biomedical nanotechnology C1 - http://www.ncbi.nlm.nih.gov/pubmed/23627048?dopt=Abstract DA - 2013 Feb DO - 10.1166/jbn.2013.1491 IS - 2 J2 - J Biomed Nanotechnol LA - eng N2 -

Vaccines play an essential role in keeping humans healthy. Innovative approaches to their use include the utilization of plasmid DNA encoding sequences to express foreign antigens. DNAhsp65 from Mycobacterium leprae is suitable for this purpose due to its ability to elicit a powerful immune response. Controlled release systems represent a promising approach to delivering vaccines. In this work, we used liposomes or PLGA systems to deliver DNAhsp65 to treat the pulmonary fungal infection Paracoccidioidomycosis. Both formulations modulated a protective immune response and reduced the pulmonary fungal burden even in the groups receiving less than four times the amount of the DNAhps65 entrapped within the nanoparticles. Although both systems had the same effective therapeutic results, the advantage of the liposome formulation was that it was administered intranasally, which may be more easily accepted by patients. These systems are a great alternative to be considered as adjuvant vaccine therapy for systemic mycosis.

PY - 2013 SP - 221 EP - 30 T2 - Journal of biomedical nanotechnology TI - Nanobiotechnological approaches to delivery of DNA vaccine against fungal infection. VL - 9 SN - 1550-7033 ER -