TY - JOUR
KW - Antigens, Bacterial
KW - Biological Assay
KW - Biomarkers
KW - Buffers
KW - Cytokines
KW - Enzyme-Linked Immunosorbent Assay
KW - Glycolipids
KW - Humans
KW - Immunity, Humoral
KW - Interferon-gamma
KW - Interleukin-10
KW - leprosy
KW - Mycobacterium Infections
KW - Reference Values
KW - Sensitivity and Specificity
AU - Corstjens P
AU - Dood CJ
AU - Ploeg-van Schip JJ
AU - Wiesmeijer K
AU - Riuttamäki T
AU - Meijgaarden K
AU - Spencer JS
AU - Tanke HJ
AU - Ottenhoff T
AU - Geluk A
AB - <p><b>OBJECTIVE: </b>The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.</p><p><b>DESIGN AND METHODS: </b>A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.</p><p><b>RESULTS: </b>Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.</p><p><b>CONCLUSIONS: </b>The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.</p>
BT - Clinical biochemistry
C1 - http://www.ncbi.nlm.nih.gov/pubmed/21763300?dopt=Abstract
DA - 2011 Oct
DO - 10.1016/j.clinbiochem.2011.06.983
IS - 14-15
J2 - Clin. Biochem.
LA - eng
N2 - <p><b>OBJECTIVE: </b>The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.</p><p><b>DESIGN AND METHODS: </b>A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.</p><p><b>RESULTS: </b>Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.</p><p><b>CONCLUSIONS: </b>The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.</p>
PY - 2011
SP - 1241
EP - 6
T2 - Clinical biochemistry
TI - Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177995/pdf/nihms310566.pdf
VL - 44
SN - 1873-2933
ER -