TY - JOUR KW - Bacteriological Techniques KW - Humans KW - leprosy KW - Molecular Diagnostic Techniques KW - Mycobacterium leprae KW - polymerase chain reaction KW - ROC Curve KW - Specimen Handling AU - Aye KS AU - Matsuoka M AU - Kai M AU - Kyaw K AU - Win AA AU - Shwe MM AU - Thein M AU - Htoo MM AU - Htoon MT AB -

The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA.

BT - Japanese journal of infectious diseases C1 - http://www.ncbi.nlm.nih.gov/pubmed/21617312?dopt=Abstract DA - 2011 DO - 10.1111/j.1744-313X.2010.00993.x IS - 3 J2 - Jpn. J. Infect. Dis. LA - eng N2 -

The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA.

PY - 2011 SP - 246 EP - 8 T2 - Japanese journal of infectious diseases TI - FTA card utility for PCR detection of Mycobacterium leprae. UR - http://www.nih.go.jp/niid/JJID/64/246.pdf VL - 64 SN - 1884-2836 ER -