TY - JOUR
KW - Animals
KW - Antigens, Bacterial
KW - BCG Vaccine
KW - Bacterial Vaccines
KW - Base Sequence
KW - DNA Primers
KW - DNA, Bacterial
KW - Interferon-gamma
KW - leprosy
KW - Mice
KW - Mycobacterium leprae
KW - T-Lymphocytes
KW - Vaccines, DNA
AU - Martin E
AU - Roche P W
AU - Triccas J A
AU - Britton W J
AB - <p>The continuing incidence of leprosy infection around the world and the inability of Mycobacterium bovis bacille Calmette-Guérin (BCG) to protect certain populations clearly indicates that an improved vaccine against leprosy is needed. The immuno dominant 35 kDa protein, shared by Mycobacterium leprae and Mycobacterium avium, but not Mycobacterium tuberculosis or BCG, is recognised by >90% of leprosy patients, making it an ideal candidate antigen for a subunit vaccine. Immunization of outbred Swiss Albino mice with a DNA-35 vaccine stimulated specific T cell activation and IFN-gamma production. DNA-35 immunization induced significant levels of protection against M. leprae footpad infection, comparable to that produced by BCG. Therefore, DNA immunization with the 35 kDa antigen is effective against M. leprae infection and genetic immunization with a combination of antigens holds the potential for an improved vaccine against leprosy.</p>
BT - Vaccine
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11163661?dopt=Abstract
DA - 2001 Jan 08
DO - 10.1016/s0264-410x(00)00374-1
IS - 11-12
J2 - Vaccine
LA - eng
N2 - <p>The continuing incidence of leprosy infection around the world and the inability of Mycobacterium bovis bacille Calmette-Guérin (BCG) to protect certain populations clearly indicates that an improved vaccine against leprosy is needed. The immuno dominant 35 kDa protein, shared by Mycobacterium leprae and Mycobacterium avium, but not Mycobacterium tuberculosis or BCG, is recognised by >90% of leprosy patients, making it an ideal candidate antigen for a subunit vaccine. Immunization of outbred Swiss Albino mice with a DNA-35 vaccine stimulated specific T cell activation and IFN-gamma production. DNA-35 immunization induced significant levels of protection against M. leprae footpad infection, comparable to that produced by BCG. Therefore, DNA immunization with the 35 kDa antigen is effective against M. leprae infection and genetic immunization with a combination of antigens holds the potential for an improved vaccine against leprosy.</p>
PY - 2001
SP - 1391
EP - 6
T2 - Vaccine
TI - DNA encoding a single mycobacterial antigen protects against leprosy infection.
VL - 19
SN - 0264-410X
ER -