TY - JOUR
KW - Bacterial Proteins
KW - Bacterial Typing Techniques
KW - Genes, Bacterial
KW - Genetic Variation
KW - Genome, Bacterial
KW - genotype
KW - Geography
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Sequence Analysis, DNA
KW - Sigma Factor
AU - Matsuoka M
AU - Maeda S
AU - Kai M
AU - Nakata N
AU - Chae G T
AU - Gillis T P
AU - Kobayashi K
AU - Izumi S
AU - Kashiwabara Y
AB - <p>The genetic diversity and related global distribution of 51 Mycobacterium leprae isolates were studied. Isolates were obtained from leprosy patients from 12 geographically distinct regions of the world and two were obtained from nonhuman sources. Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae. Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. Isolates from Japan (except Okinawa) and Korea belonged to one group, while those from Southeast Asian countries, Brazil, Haiti and Okinawa in Japan belonged to a second genotype. M. leprae obtained from two nonhuman sources (an armadillo and a mangabey monkey) revealed the latter genotype. These results demonstrate the genetic diversity of M. leprae and the related genotype-specific distribution in the world.</p>
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11036491?dopt=Abstract
DA - 2000 Jun
IS - 2
J2 - Int. J. Lepr. Other Mycobact. Dis.
LA - eng
N2 - <p>The genetic diversity and related global distribution of 51 Mycobacterium leprae isolates were studied. Isolates were obtained from leprosy patients from 12 geographically distinct regions of the world and two were obtained from nonhuman sources. Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae. Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. Isolates from Japan (except Okinawa) and Korea belonged to one group, while those from Southeast Asian countries, Brazil, Haiti and Okinawa in Japan belonged to a second genotype. M. leprae obtained from two nonhuman sources (an armadillo and a mangabey monkey) revealed the latter genotype. These results demonstrate the genetic diversity of M. leprae and the related genotype-specific distribution in the world.</p>
PY - 2000
SP - 121
EP - 8
T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
TI - Mycobacterium leprae typing by genomic diversity and global distribution of genotypes.
UR - http://ila.ilsl.br/pdfs/v68n2a01.pdf
VL - 68
SN - 0148-916X
ER -