TY - JOUR KW - Antigen Presentation KW - Antigens KW - Antigens, Bacterial KW - Antigens, CD1 KW - Antigens, Surface KW - CD4 Antigens KW - CD4-Positive T-Lymphocytes KW - Cell Membrane KW - Cells, Cultured KW - Glycolipids KW - Humans KW - Lectins, C-Type KW - leprosy KW - Lipopolysaccharides KW - Mycobacterium leprae KW - Mycolic Acids KW - NK Cell Lectin-Like Receptor Subfamily B KW - Peptides KW - Protein Biosynthesis KW - Proteins KW - Receptors, Immunologic KW - T-Lymphocyte Subsets AU - Sieling P A AU - Ochoa M T AU - Jullien D AU - Leslie D S AU - Sabet S AU - Rosat J P AU - Burdick A E AU - Rea T H AU - Brenner M B AU - Porcelli S A AU - Modlin R L AB -

Both the CD4-CD8- (double negative) and CD4-CD8+ T cell lineages have been shown to contain T cells which recognize microbial lipid and glycolipid Ags in the context of human CD1 molecules. To determine whether T cells expressing the CD4 coreceptor could recognize Ag in the context of CD1, we derived CD4+ T cell lines from the lesions of leprosy patients. We identified three CD4+ Mycobacterium leprae-reactive, CD1-restricted T cell lines: two CD1b restricted and one CD1c restricted. These T cell lines recognize mycobacterial Ags, one of which has not been previously described for CD1-restricted T cells. The response of CD4+ CD1-restricted T cells, unlike MHC class II-restricted T cells, was not inhibited by anti-CD4 mAb, suggesting that the CD4 coreceptor does not impact positive or negative selection of CD1-restricted T cells. The CD4+ CD1-restricted T cell lines produced IFN-gamma and GM-CSF, the Th1 pattern of cytokines required for cell-mediated immunity against intracellular pathogens, but no detectable IL-4. The existence of CD4+ CD1-restricted T cells that produce a Th1 cytokine pattern suggests a contributory role in immunity to mycobacterial infection.

BT - Journal of immunology (Baltimore, Md. : 1950) C1 - http://www.ncbi.nlm.nih.gov/pubmed/10779786?dopt=Abstract DA - 2000 May 01 DO - 10.4049/jimmunol.164.9.4790 IS - 9 J2 - J. Immunol. LA - eng N2 -

Both the CD4-CD8- (double negative) and CD4-CD8+ T cell lineages have been shown to contain T cells which recognize microbial lipid and glycolipid Ags in the context of human CD1 molecules. To determine whether T cells expressing the CD4 coreceptor could recognize Ag in the context of CD1, we derived CD4+ T cell lines from the lesions of leprosy patients. We identified three CD4+ Mycobacterium leprae-reactive, CD1-restricted T cell lines: two CD1b restricted and one CD1c restricted. These T cell lines recognize mycobacterial Ags, one of which has not been previously described for CD1-restricted T cells. The response of CD4+ CD1-restricted T cells, unlike MHC class II-restricted T cells, was not inhibited by anti-CD4 mAb, suggesting that the CD4 coreceptor does not impact positive or negative selection of CD1-restricted T cells. The CD4+ CD1-restricted T cell lines produced IFN-gamma and GM-CSF, the Th1 pattern of cytokines required for cell-mediated immunity against intracellular pathogens, but no detectable IL-4. The existence of CD4+ CD1-restricted T cells that produce a Th1 cytokine pattern suggests a contributory role in immunity to mycobacterial infection.

PY - 2000 SP - 4790 EP - 6 T2 - Journal of immunology (Baltimore, Md. : 1950) TI - Evidence for human CD4+ T cells in the CD1-restricted repertoire: derivation of mycobacteria-reactive T cells from leprosy lesions. UR - http://www.jimmunol.org/content/164/9/4790.full.pdf+html VL - 164 SN - 0022-1767 ER -