TY - JOUR KW - Bacterial Proteins KW - Cell Division KW - Cells, Cultured KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Immunodominant Epitopes KW - Leprosy, Tuberculoid KW - Membrane Proteins KW - Nontuberculous Mycobacteria KW - T-Lymphocytes AU - Mehrotra J AU - Mittal A AU - Dhindsa M S AU - Sinha S AB -

Integral membrane proteins (IMP) represent a serologically distinct class of mycobacterial antigens which are potent stimulators of human T cells (Mehrotra et al., Clin Exp Immunol 1995; 102:626). The range of IMP from Mycobacterium fortuitum was resolved by continuous elution SDS-PAGE to recover 31 discrete fractions covering bands up to approximately 58 kD. The fractions, after removal of SDS, were subjected to human T cell proliferation assays for the identification of immunodominant molecule(s). A low molecular weight (<20kD) fraction was able to stimulate T cells from 11 out of 12 donors comprising mainly tuberculoid leprosy patients. The described protocol is well suited to situations where large quantities of antigenic protein mixtures must be processed in order to get the purified molecules/fractions in amounts required for immunoepidemiological studies.

BT - Clinical and experimental immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/9328120?dopt=Abstract DA - 1997 Sep DO - 10.1046/j.1365-2249.1997.4531351.x IS - 3 J2 - Clin. Exp. Immunol. LA - eng N2 -

Integral membrane proteins (IMP) represent a serologically distinct class of mycobacterial antigens which are potent stimulators of human T cells (Mehrotra et al., Clin Exp Immunol 1995; 102:626). The range of IMP from Mycobacterium fortuitum was resolved by continuous elution SDS-PAGE to recover 31 discrete fractions covering bands up to approximately 58 kD. The fractions, after removal of SDS, were subjected to human T cell proliferation assays for the identification of immunodominant molecule(s). A low molecular weight (<20kD) fraction was able to stimulate T cells from 11 out of 12 donors comprising mainly tuberculoid leprosy patients. The described protocol is well suited to situations where large quantities of antigenic protein mixtures must be processed in order to get the purified molecules/fractions in amounts required for immunoepidemiological studies.

PY - 1997 SP - 446 EP - 50 T2 - Clinical and experimental immunology TI - Fractionation of mycobacterial integral membrane proteins by continuous elution SDS-PAGE reveals the immunodominance of low molecular weight subunits for human T cells. VL - 109 SN - 0009-9104 ER -